Objective To investigate the resistance of Musca domestica to commonly used insecticides in Shaoxing city, Zhejiang province, provide guidance in the control of M. domestica. Methods Musca domestica were collected from the main district of Shaoxing during August-September 2017. WHO recommended topical application method was used to determine the resistance levels of M. domestica, which were the age of 4-5 d after eclosion, to five insecticides (propoxur, DDVP, β-cypermethrin, permethrin, deltamethrin). Results Musca domestica in Shaoxing city showed different levels of resistance to the five insecticides. Statistics revealed that the resistance to propoxur could not obtain results by using topical application method. The median lethal dose (LD₅₀) of the adult flies against deltamethrin, DDVP, β-cypermethrin and permethrin were 0.064 64, 3.117 46, 0.231 42, and 0.180 47 μg/♀, and the resistance ratios were 71.82, 68.82, 64.28, and 16.71-fold, respectively; the resistance to deltamethrin was the highest. Conclusion Musca domestica in Shaoxing city have developed resistance to five insecticides in different levels. Insecticides resistance surveillance should be highlighted for rational use of insecticides.