Objective To investigate and evaluate the constructive process and effect of vector control which based on the construction of "mosquito-free village" in Xuexiazhuang village, Pujiang, Zhejiang province, and then to provide experience for mosquito control in rural areas. Methods The adult and larval densities were monitored with lamp traps and 100-household index respectively from April to October in each year from 2016 to 2017. A random sampling method was adopted to select 50 households in pilot and control villages from which one family member was surveyed. The face-to-face questionnaire was about the anti-mosquito related knowledge, attitude and behaviors. Examination and evaluation of construction mode of "mosquito-free village" was deeply explored via the method of interview, data review, on-site observation. Results Monitoring results of Xuexiazhuang village from April to October 2017 showed that the adult mosquito density was 0.50, 0.50, 0, 0, 0.75, 0, 0 individual per lamp trap per night, respectively, and the 100-household index was 18.00, 4.00, 4.00, 2.00, 0, 2.00, 2.00. The awareness rates of anti-mosquito and correct behavior formation reached 82.80%, higher than that in the control village (67.80%). The construction of "mosquito-free village" is much more institutionalized and standardized than before, and the long-term mechanism has been formed. Conclusion The mode of rural mosquito control based on "mosquito-free village" can effectively reduce the density of mosquitoes. Formation of institutionalized, standardized long-term mechanism of "mosquito-free village" construction work may provide reference for a much more scientific, effective, low cost and sustainable development of vector control in rural areas in the future.