Objective Tick-borne pathogens were detected from adult ticks collected in Manchuria port areas on the borders between China, Russia and Mongolia during 2012-2014, to provide basis for monitoring and control of local ticks and tick-borne diseases. Methods The free ticks were collected in the grass and mixed forest areas by hourly flagging method, and parasitic ticks from wild gazelles and other animals by body surface inspection method. RT-PCR was proceeded to detect tick-borne encephalitis virus(TBEV), Crimean-Congo virus(CCHFV), Novel Bunyavirus(NTB); PCR was proceeded to detect Borrelia burgdorferi, Rickettsia, Francisella tularensis, Bartonella, Anaplasma phagocytophilum, Babesia, Coxiella burneti from RNA and DNA derived from ticks, respectively. Results A total of 826 adult ticks were collected and identified as Ixodes persuleatus and Dermacentor nuttalli. PCR results for F. tularensis and Babesia spp. were all negative, B. burgdorferi, A. phagocytophilum, Bartonella, CCHFV, NTB, TBEV were identified with statistically significant differences between I. persuleatus and D. nuttalli(χ2=10.022, 15.776, 16.448, 21.789, 16.448, 16.871, all the P=0.000). Positive results showed as 5.15%(20/388) infection rate from 43 parasitic ticks and 345 free ticks. Borrelia burgdorferi was identified by PCR with statistically significant differences between parasitic and free ticks(χ2=24.617, P=0.000). Conclusion These findings showed the activities of tick-borne pathogens in Manchuria port, different pathogens were carried by different ticks and was common to see co-infection in ticks. It is necessary to strengthen tick-borne disease surveillance and control in this region.
YANG Yu, GAO Yan-fei, CAO Yang, WANG Jing, DENG Hua, WANG Jian-cheng, CHEN Xue-song, LIU Li-juan, XU Bao-liang
. Investigation on tick-borne pathogens in Inner Mongolia Manchuria port area during 2012-2014[J]. Chinese Journal of Vector Biology and Control, 2018
, 29(2)
: 147
-150
.
DOI: 10.11853/j.issn.1003.8280.2018.02.008
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