Objective To analyze the ferritin gene expression levels in different strains of Culex pipiens pallens. Methods The primers were designed and synthesized, target gene was amplified by fluorescent quantitative PCR to detect the expression level between resistant strain and susceptible strains of Cx. pipiens pallens. A t test method was used to detect the statistical significance for the two samples. Results The ferritin gene of Cx. pipiens pallens was cloned successfully. The quantitative average of ferritin fluorescence was 11.41 in susceptible strain and 15.73 in resistant strain; the quantitative average of reference gene fluorescence was 10.03 in susceptible strain, and 18.27 in resistant strain. Fluorescent quantitative PCR proved the ferritin gene expression level of resistant strain was 15.08 times that of susceptible strain, t test showed that two samples of ferritin gene expression differences were statistically significant(t=26.100, P < 0.001). Conclusion Culex pipiens pallens ferritin gene can be used as a new gene target for resistant detection and management.
LU Jing, SHI Qi-qi, CHENG Peng, GONG Mao-qing, CUI Wen, TAN Wen-bin
. Comparative analysis on expression levels of the ferritin gene in Culex pipiens pallens[J]. Chinese Journal of Vector Biology and Control, 2018
, 29(2)
: 134
-137
.
DOI: 10.11853/j.issn.1003.8280.2018.02.005
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