Objective To determine the effect of diflubenzuron on dengue vector Aedes mosquitoes and provide evidence for mosquito resistance management and vector control. Methods WHO recommended method for insect growth regulators was utilized; the resistant Aedes strains were collected from Jinghong city the dengue fever epidemic area, the data were analyzed with SPSS 24.0 to create a log-probit model to determine the IE₅₀ of the susceptible strains and resistant strains, calculate the resistance ratio, observe the the stage-and concentration-dependent mortality, and analyze the characteristics of diflubenzuron against Aedes mosquitoes. Results The IE_50s of the resistant strains of Ae. aegypti and Ae. albopictus were 0.002 53(95%CI:0.002 16-0.002 89) μg/ml and 0.002 33(95%CI:0.001 98-0.003 12) μg/ml, the resistant ratios were 1.77-and 1.71-fold, indicating that no cross resistance between diflubenzuron and pyrethroid. The mortality peak of Ae. albopictus and Ae. aegypti was 4-6 d post-exposure, indicating that the effect of diflubenzuron was slow as compared with commonly used conventional insecticides. Under the different concentrations, the effect was different. The low concentration mainly resulted mortality at adult stage, whilethe high concentration mostly impacted on pupal and/or larval stages. Conclusion The resistant strains of Ae. albopictus and Ae. aegypti were susceptible to diflubenzuron. The recommended dose was 0.006 μg/ml,but the lethal effect of diflubenzuron was slow. It can be used as a selective insecticide for resistance management and vector control.