Detection of the I1532 and F1534 kdr mutations and a novel mutant allele I1532T in VGSC gene in the field populations of Aedes albopictus from China

CHEN Han-ming, GAO Jing-peng, JIANG Jin-yong, PENG Heng, MA Ya-jun

doi:10.11853/j.issn.1003.8280.2018.02.002

Chinese Journal of Vector Biology and Control >

2018 , Vol. 29 >Issue 2: 120 - 125

DOI: https://doi.org/10.11853/j.issn.1003.8280.2018.02.002

Detection of the I1532 and F1534 kdr mutations and a novel mutant allele I1532T in VGSC gene in the field populations of Aedes albopictus from China

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1 Department of Tropical Diseases, Department of Medical Microbiology and Parasitology, Basic Medical College Navy Military Medical University, Shanghai 200433, China;
2 Yunnan Institute of Parasitic Diseases

Received date: 2018-01-18

Online published: 2018-04-20

Supported by

Supported by the National Natural Science Foundation of China(No. 81371848)and the National Science and Technology Major Project of China(No. 2017ZX10303404002001)

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Abstract

Objective To detect the kdr gene mutations in the field populations of Aedes albopictus from China and preliminarily clarify its adaptive evolution. Methods The mosquito larvae and pupae were dip-samples from breeding sites from Yangpu district in Shanghai, Nanjing city in Jiangsu province, Hangzhou city in Zhejiang province, and Jinghong city in Yunnan province, in China, and brought back to insectary to rise till adults. The mosquito adults were also collected directly in the fields using BG-trap or sucking tube. The single individual genomic DNA of Ae. albopictus was extracted after species identification. The VGSC gene fragments were amplified by PCR, which were sequenced and analyzed for detection of the kdr mutations. The genotypic linkage disequilibrium and Hardy-Weinberg equilibrium(HWE) was tested by GENPOP software. Results A total of 299 individuals collected from four field populations of Ae. albopictus were analyzed. There were two positions showed mutations including codon 1532 and 1534 of kdr gene. Two alleles at codon 1532 were wildtype ATC/I and mutant ACC/T, with frequencies 91.30% and 8.70%, respectively. Five alleles at codon 1534 were wildtype TTC/F, mutant allele TCC/S, TCG/S, TTG/L, and TGC/C, with frequencies of 45.99%, 52.01%, 0.33%, 1.00%, and 0.67%, respectively. There were 27 individuals(9.03%) with both mutations in 1532 and 1534 at the same time. The test results showed that the population conformed to HWE(P > 0.001), and the two loci were genetic linkage (P < 0.01). Conclusion This study first reported the novel mutant allele I1532T, and found a mutant allele TCG/S at the codon 1534 of Ae. albopictus in China. More important is a new challenge that an individual harbors two codons of simultaneous mutations. The results suggested that they should be resistant to pyrethroid insecticides. The multiple site mutations may be resulted from the selection pressure of insecticides.

Key words： Aedes albopictus; Knockdown resistance gene; Gene mutation; Resistance

Cite this article

CHEN Han-ming, GAO Jing-peng, JIANG Jin-yong, PENG Heng, MA Ya-jun . Detection of the I1532 and F1534 kdr mutations and a novel mutant allele I1532T in VGSC gene in the field populations of Aedes albopictus from China[J]. Chinese Journal of Vector Biology and Control, 2018 , 29(2) : 120 -125 . DOI: 10.11853/j.issn.1003.8280.2018.02.002

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