Objective To understand whether there is a drug-or disinfectant-resistant strains in natural plague foci in Tibet, and provide the accurate information for clinical treatment of plague. Methods According to the aminoglycoside resistant gene of streptomycin resistant, StrB, StrA, beta lactam antibiotics TEM, SHV, and CTX-M gene, sulfamilamide resistant Sul1, Sul2, and Sul3, and anti-disinfectant QacEdeltal-sul1 gene sequence the National Center for Biotechnology Information (NCBI) released,a pair of primers in each gene was designed separately. DNA of strains isolated from natural plague foci in Tibet were amplified by PCR using every pair of primers. Results Negative and positive control were established, samples by PCR amplification results were negative, there were no streptomycin, sulfamilamide and beta lactam antimicrobial drug resistance genes and anti-disinfectant genes in strains studied. Conclusion The Tibet autonomous region of natural plague foci did not appear to have drug-or disinfectant-resistant Yersina pestis.
HE Jian, YANG Xiao-yan, LI Sheng, JIN Juan, ZHANG Qi, XIN You-quan, JIN Yong, XIONG Hao-ming, YANG Han-qing, WEI Bai-qing, DAI Rui-xia, QI Zhi-zhen
. Study on the drug and disinfectant resistant genes of Yersina pestis in natural plague foci in Tibet[J]. Chinese Journal of Vector Biology and Control, 2018
, 29(1)
: 61
-63
.
DOI: 10.11853/j.issn.1003.8280.2018.01.015
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