Objective To understand the dynamic changes of trehalose and trehalase of Anopheles sinensis. Methods Enzyme-linked immunosorbent assay (ELISA) was used to detect trehalose and trehalase contents of female adult. ANOVA was used to determine the statistical significance of trehalose and trehalase contents. Subsequently, Tukey's multiple comparison was applied for significance analysis. Results The results showed that the trehalose and trehalase of female An. sinensis increased first and decreased there after post adult emergence. The females after oviposition had lower levels of trehalose and trehalase contents. The contents of trehalose which reached the peak approximately on the second day in adult stage[(2.658 5±0.116 5) mg/g] were significantly different (F=21.383, df=100, P<0.05). However, the contents of trehalase reached the peak stage approximately on the third day in adult stage[(0.111 6±0.002 5) μmol/g]. Conclusion The trehalose and trehalase contents of female An. sinensis have a regular pattern, which is related to the energy accumulation and consumption.
LIU Li-juan, HU Ying-xin, LI Ya-ping, CHENG Peng, GUO Xiu-xia, GONG Mao-qing
. Dynamic changes of trehalose and trehalase in female Anopheles sinensis Wiedemann (Diptera: Culicidae)[J]. Chinese Journal of Vector Biology and Control, 2018
, 29(1)
: 50
-52
.
DOI: 10.11853/j.issn.1003.8280.2018.01.013
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