Study of identification methods for arthropod bloodmeal donor based on molecular biology

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  • Zhejiang Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang Province, China

Received date: 2017-06-15

  Online published: 2017-12-20

Supported by

Supported by the Medicine and Health Science and Technology Plan Project of Zhejiang Province(No. 2017KY291)

Abstract

Hematophagous arthropods are important vectors for vector-borne diseases. The identification of hematophagous arthropods'hosts is critical to exploration of vector-borne disease ecology and further control measures. The strategy of bloodmeal identification is effective to ascertain arthropod hosts. With the development of molecular biology technology, DNA-based methods, which include PCR, DNA sequencing, restriction fragment length polymorphism, reverse line blot hybridization, and DNA fingerprinting, enhanced the specificity and sensitivity of hematophagous arthropod bloodmeal identification greatly. This paper reviewed the principles, advantages and disadvantages of molecular protocols for detection of host DNA, which might help to provide reference for the prevention and control of related vector-borne diseases.

Cite this article

LIU Ying, SUN Ji-min, LING Feng, LU Miao-gui, SHI Xu-guang, REN Jiang-ping, ZHANG Rong, CHEN En-fu . Study of identification methods for arthropod bloodmeal donor based on molecular biology[J]. Chinese Journal of Vector Biology and Control, 2017 , 28(6) : 603 -607 . DOI: 10.11853/j.issn.1003.8280.2017.06.025

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