Objective By investigating the positive rate of F1 antibody in indicator animals in the quiescent stage of domestic rodent-maintained epidemic focus of plague in Yunnan province, the hidden epidemic situation and dynamic in the quiescent stage of plague were discussed. Methods From November 11, 2015 to July 22, 2016, the sample collection of plague indicator animals was carried out in Lancang, Midu, Yiliang, and Lianghe counties in Yunnan province, and employed the IHA laboratory testing of F1 antibody for the serum-collection of plague indicator animals (dog/cat). Results In four historical epidemic areas of plague of Lancang, Midu,Yiliang, and Lianghe counties in Yunnan province, 393 samples of animal sera were collected, including 387 dogs and 6 cats. The serum-collections of plague indicator animals'F1 antibody detection tested all negative. Conclusion The possibility of the plague epidemic in this region was meager, and sero-epidemiological survey of indicator animals in the quiescent stage makes a huge difference in the plague prevention and control work.