Objective To investigate the resistance of Aedes aegypti and Ae. albopictus to insecticides in Ruili city, Yunnan province, to provide the basis for integrated control of dengue vector. Methods Larvae of Ae. aegypti and Ae. albopictus were collected in Ruili city and Jiegao port and reared in laboratory to get F1 generation, to test their susceptibility to different kinds of insecticides by bioassays. The resistance level was decided by adjusted mortality. Results The 24 h mortality rate of adult Ae. aegypti in Ruili city to 0.25% permethrin, 0.03% λ-cyhalothrin, 8.00% α-cypermethrin, 0.80%malathion, 0.25% fenitrothion, 0.10% propoxur, and 4.00% DDT were 1.40% (resistant), 20.12% (resistant), 80.31% (suspicious resistant), 100% (susceptible),100% (susceptible), 100% (susceptible) and 14.93% (resistant)respectively. The 24 h mortality rate of adult Ae. aegypti in Jiegao port to above insecticides were 0.55% (resistant), 2.13% (resistant), 62.35% (resistant), 100% (susceptible), 100% (susceptible), 100% (susceptible) and 0 (resistant) respectively. In addition, the 24 h mortality of adult Ae. albopictus in Ruili city to 0.75% permethrin, 1.80% λ-cyhalothrin, 8.00% α-cypermethrin, 0.10% deltamethrin, 3.00% beta-cypermethrin, 0.44% malathion, 0.18% fenitrothion and 0.06% propoxur were 8.11% (resistant), 83.85% (suspicious resistant), 75.18% (resistant), 4.97% (resistant), 89.13% (suspicious resistant), 99.23% (susceptible), 100% (susceptible) and 100% (susceptible) respectively. Conclusion Aedes aegypti and Ae. albopictus in Ruili city have developed different levels resistance to pyrethroid insecticides, but present high susceptible to malathion, fenitrothion and propoxur.
LAN Xue-mei, ZHENG Yu-ting, DONG Chao-liang, LIU Yong-hua, YIN Xiao-xiong, YANG Ming-dong, JIANG Jin-yong
. Investigation on the resistance of Aedes aegypti and Ae. albopictus to several insecticides in Ruili city, Yunnan province[J]. Chinese Journal of Vector Biology and Control, 2017
, 28(6)
: 572
-575
.
DOI: 10.11853/j.issn.1003.8280.2017.06.014
[1] 谢晖, 周红宁, 杨亚明. 我国登革热重要媒介埃及伊蚊的研究进展[J]. 中国媒介生物学及控制杂志, 2011, 22(2):194-197.
[2] 安继尧,严格,张学文,等. 白纹伊蚊:登革热的重要媒介[J]. 医学动物防制, 2003, 17(8):449-452.
[3] 林钟宇,潘华峰,王正,等. 2014年广东登革热流行趋势与防控对策[J]. 卫生软科学, 2015, 29(9):590-592.
[4] 刘振江,吴春敏,叶素贞,等. 福建北部首次登革热暴发疫情分子流行病学研究[J]. 中国病毒病杂志, 2016, 6(1):54-58.
[5] 许国章,施南峰,董红军,等. 浙江慈溪输入性登革热暴发的流行特征与防制对策研究[J]. 中国热带医学, 2006, 6(7):1129-1131.
[6] 李华昌,杨贵荣. 云南临沧市首次本地感染登革热流行病学调查[J]. 中国热带医学, 2009, 9(10):2013-2014.
[7] 董学书,蔡福昌,周红宁,等. 云南省边境口岸蚊类调查[J]. 中国媒介生物学及控制杂志, 2004, 15(2):142-145.
[8] 董书华,番绍虎,马丽,等. 云南省芒市埃及伊蚊分布调查[J]. 中国媒介生物学及控制杂志, 2011, 22(6):592-594.
[9] Oo TT,Storch V,Madon MB,et al. Factors influencing the seasonal abundance of Aedes (Stegomyia) aegypti and the control strategy of dengue and dengue haemorrhagic fever in Thanlyin Township, Yangon city, Myanmar[J]. Trop Biomed, 2011, 28(2):302-311.
[10] Thu HM,Lowry K,Myint TT,et al. Myanmar dengue outbreak associated with displacement of serotypes 2, 3,and 4 by dengue 1[J]. Emerg Infect Dis, 2004, 10(4):593-597.
[11] NgweTun MM,Kyaw AK,Makki N,et al. Characterization of the 2013 dengue epidemic in Myanmar with dengue virus 1 as the dominant serotype[J]. Infect Genet Evol, 2016, 43:31-37.
[12] 刘永华,尹小雄,杨召兰,等. 云南省瑞丽市2013年登革热暴发的流行病学分析[J]. 中国媒介生物学及控制杂志, 2014, 25(6):524-526.
[13] 韩继周,尹正留,刘永华. 2014年瑞丽市登革热疫情流行特征分析[J]. 中国卫生产业, 2015(15):186-188.
[14] 姜进勇,郭晓芳,唐烨榕,等. 云南省2004-2014年输入性登革热病例监测与防控对策分析[J]. 中国媒介生物学及控制杂志, 2016, 27(1):5-8.
[15] 杨捷,张保森,唐发良,等. 一起登革热暴发疫情流行病学特征分析与现场处置[J]. 中国病原生物学杂志, 2014, 9(11):1020-1024.
[16] 董学书,周红宁,龚正达. 云南蚊类志[M]. 昆明:云南科技出版社, 2010:60-82.
[17] 王义冠,师灿南,林国松,等. 广东省潮州市白纹伊蚊对常用杀虫剂的抗药性[J]. 中国媒介生物学及控制杂志, 2016, 27(3):228-231.
[18] Abbott WS. A method of computing the effectiveness of an insecticide[J]. J Enon Entomol, 1925, 18(2):265-267.
[19] World Health Organization. Insecticide resistance in mosquito vectors of disease:report of a regional working groupmeeting, Salatiga (Indonesia)[R]. New Delhi:World Health Organization, 1997:6.
[20] 李海龙,张桂林,党荣理,等. 应用SPSS软件进行杀虫剂的KT50分析[J]. 中国媒介生物学及控制杂志, 2011, 22(2):155-157.
[21] Li CX,Kaufman PE,Xue RD,et al. Relationship between insecticide resistance and kdr mutations in the dengue vector Aedes aegypti in Southern China[J]. Parasit Vector, 2015, 8:325.
[22] Kawada H,Oo SZM,Thaung S,et al. Co-occurrence of point mutations in the voltage-gated sodium channel of pyrethroid-resistant Aedes aegypti populations in Myanmar[J]. PLoS Negl Trop Dis, 2014, 8(7):e3032.
[23] Thanispong K, Sathantriphop S, Chareonviriyaphap T. Insecticide resistance of Aedes aegypti and Culex quinquefasciatus in Thailand[J]. J Pestic Sci, 2008, 33(4):351-356.
[24] 黄慕嫦,刘鹰航,区博文,等. 江门市白纹伊蚊对5种杀虫剂的抗性监测与抗性治理对策[J]. 中华卫生杀虫药械, 2016, 22(2):142-144.
[25] 孟凤霞,王义冠,冯磊,等. 我国登革热疫情防控与媒介伊蚊的综合治理[J]. 中国媒介生物学及控制杂志, 2015, 26(1):4-10.
[26] 亢春雨,赵春青,吴刚. 昆虫抗药性分子机制研究的新进展[J]. 华东昆虫学报, 2007, 16(2):136-140.