Objective To assess and prevent the risks caused by vectors and the vector-borne disease during 2016 International Horticultural Exposition. Methods Judging the hazard caused by vectors and the risk classification of vector-borne diseases by risk assessment index matrix method and Borda sequence value method. Results It was high risk for the vector biting and infestation and food poisoning caused by vectors, it was middle risk for the cable damage and communication and power facility disruption. The prevalence of Japanese encephalitis (JE), malaria, plague, and hemorrhagic fever with renal syndrome (HFRS) were at high risk. The prevalence of dengue fever, yellow fever, filariasis, Zika virus disease, leptospirosis, murine typhus, and tsutsugamushi disease were at middle risk. Food poisoning caused by vectors ranked the first in vector harm by Borda sequence value method, vector biting and infestation and communication and power facility disruption by vectors ranked the second. JE, malaria, and HFRS ranked the first in vector-borne diseases. Dengue fever, yellow fever, filariasis, Zika virus disease, leptospirosis, murine typhus, and tsutsugamushi disease ranked the forth. Plague ranked the eleventh. Conclusion The integrated application of risk matrix and Borda count is effective in assessing the risk levels of the hazards caused by vectors and the vector-borne disease, and therefore is worthy of wide promotion.