Objective To rapidly evaluate the palatability of poison baits to domestic rodents, and to provide basic evidence for procuring typical poison baits for autumn rodent control in urban areas. Methods In the middle of August, the domestic rats in dominant habitats were identified as experimental subjects who were captured from places such as livestock farms, supermarkets, department of stores, restaurants and residential areas, then rats were used to rapidly determine the feeding situation for further evaluating palatability of poison baits through a stimulated field in laboratory. Results Poisonous baits consumption from experimental group No. 1 to No. 6 were at 112.81, 113.40, 116.72,101.76, 126.89, and 130.99 g in sequence, at the meantime, non-poisonous baits consumptions were at 120.24, 104.94, 117.19, 136.40, 125.14, and 128.96 g. Besides, feeding coefficients were at 0.94, 1.08, 1.00, 0.75, 1.01, and 1.02 respectively with all palatability in A level. Conclusion All 6 poison baits have a good palatability, and a combination of indexes like price, toxicity and safety should be considered to select one or several poison baits for urban rodent control in autumn. Thus, this application research can quickly evaluate the palatability of poison baits, and is suitable for promoting the development of rodent control to centers for disease control and prevention in levels of counties or cities.