Objective To understand the genetic type and characteristics of Hantavirus carried by Rattus norvegicus in Qinhuangdao port. Methods Real-time PCR and sequencing technology were applied to detect the nucleic acid of Hantavirus. And the homologous and phylogenetic analysis of positive sequence were also accomplished. Results One of the R. norvegicus showed positive result for Hantavirus. Homogeneous analysis demonstrated that the nucleic acid sequence of Hantavirus S gene was high identity with Seoul(SEO)virus from both home and abroad. Phylogenetic analysis showed that Qinhuangdao Hantavirus was clustering together with S3 sub- genotype of Seoul(SEO)virus, and was most closely related to the isolate from Liaoning province. Conclusion The genotype of hantavirus carried by R. norvegicus in Qinhuangdao is SEO virus and the sub-genotype is S3 which is consistent with the popular type of hantavirus in Hebei province.
LI Yun, SHI Ling-li, LIU Jian-nan, YAN Ji-huan, NIE Wei-zhong, LIU Shu-ping
. S gene sequence analysis of Hantavirus carried by the Rattus norvegicus from Qinhuangdao port[J]. Chinese Journal of Vector Biology and Control, 2017
, 28(5)
: 487
-489
.
DOI: 10.11853/j.issn.1003.8280.2017.05.021
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