Objective To understand Wolbachia infection and its genotype in the cockroaches at Zhongshan area. Methods Cockroaches were collected in Zhongshan of Guangdong province between June 2015 to July 2016. wsp genes of Wolbachia were amplified and sequenced to identify if the cockroaches were infected or not, phylogenetic analysis of the wsp in these cockroaches was conducted as well. Paired t test was applied to the analysis of infection difference between the females and males. Results A total of 42 cockroaches belonging to 5 species were infected with Wolbachia out of all the studied 154 cockroach individuals of 12 species, the infection rate of Wolbachia was 27.27% at individuals level, and the difference of Wolbachia infection rate between males and females wasn't significant. There were 8 wsp haplotype sequences were classified as wAlbB, wAlbD, and wAlbF based on the phylogenetic analysis. Conclusion Wolbachia infection in Lupparia vilis, Malaccina sinica, Margatta sp.1, Margatta sp.2, and Ectobiidae sp. were detected for the first time in the world, those genotypes belong to wAlbB, wAlbD, and wAlbF. Among those wsp haplotype sequences, there might be a new wAlbF Wolbachia type in L. vilis.
CHEN Pei, CHEN Jian, QIU De-yi, HE Qiu-xing
. Monitoring and analysis of symbiotic Wolbachia in cockroaches at Zhongshan area[J]. Chinese Journal of Vector Biology and Control, 2017
, 28(5)
: 422
-427
.
DOI: 10.11853/j.issn.1003.8280.2017.05.003
[1] 郭颂,凌锋,王金娜,等. 浙江省中东部白纹伊蚊共生菌沃尔巴克氏体的调查研究[J]. 中国媒介生物学及控制杂志, 2015,26(6):545-549.
[2] Hertig M,Wolbach SB. Studies on rickettsia-like micro-organisms in insects[J]. J Med Res,1924,44(3):329-374.
[3] Hilgenboecker K,Hammerstein P,Schlattmann P,et al. How many species are infected with Wolbachia?-a statistical analysis of current data[J]. FEMS Microbiol Lett,2008,281(2):215-220.
[4] Zug R,Hammerstein P. Still a host of hosts for Wolbachia:analysis of recent data suggests that 40% of terrestrial arthropod species are infected[J]. PLoS One,2012,7(6):e38544.
[5] Baldo L,Hotopp JCD,Jolley KA,et al. Multilocus sequence typing system for the endosymbiont Wolbachia pipientis[J]. Appl Environ Microb,2006,72(11):7098-7110.
[6] Zhou WG,Rousset F,O'Neill S. Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences[J]. P Proc R Soc B:Biol Sci,1998,265(1395):509-515.
[7] Glowska E,Dragun-Damian A,Dabert M,et al. New Wolbachia supergroups detected in quill mites(Acari:Syringophilidae)[J]. Infect Genet Evol,2015,30:140-146.
[8] Sinkins SP. Wolbachia and cytoplasmic incompatibility in mosquitoes[J]. Insect Biochem Mol Biol,2004,34(7):723-729.
[9] 潘雪红,陈科伟,吕燕青,等. Wolbachia对赤眼蜂的性别调控机制及生理影响[J]. 昆虫知识,2007,44(1):32-36.
[10] 金俊. 国际航行船舶传带医学媒介生物(蜚蠊)风险评估体系研究[D]. 南京:南京理工大学,2013.
[11] Vaishampayan PA,Dhotre DP,Gupta RP,et al. Molecular evidence and phylogenetic affiliations of Wolbachia in cockroaches[J]. Mol Phylogenet Evol,2007,44(3):1346-1351.
[12] 金小宝,褚夫江,朱家勇. 德国小蠊共生菌沃尔巴克氏体的分子检测与分析[J]. 中国媒介生物学及控制杂志,2008,19(2):121-122.
[13] 岳巧云. 实用蜚蠊彩色图鉴[M]. 广州:暨南大学出版社, 2015:21-151.
[14] Werren JH,Baldo L,Clark ME. Wolbachia:master manipulators of invertebrate biology[J]. Nat Rev Microbiol,2008,6(10):741-751.
[15] 郑雅. Wolbachia诱导昆虫宿主细胞质不亲和的分子机理研究[D]. 武汉:华中师范大学,2012.
[16] Jiggins FM,von der Schulenburg JHG,Hurst GDD,et al. Recombination confounds interpretations of Wolbachia evolution[J]. Proc Roy Soc B:Biol Sci,2001,268(1474):1423-1427.
