Objective To understand the polymorphisms of D-loop gene from Mus musculus collected in Jiangsu ports, and provide support for the species identification of rodents from frontier port. Methods The D-loop genes from M. musculus were amplified, sequenced, aligned, analyzed genetic polymorphism and created genetic evolutionary tree. Results Twenty-one D-loop gene sequences were obtained from M. musculus, all of M. musculus shared 17 haploid type, 533 loci were measured in every sequence. There were 488 conservative loci and 41 mutation loci, the nucleotide differences number was 14.752 38 on average. The genetic evolutionary tree was created for 21 M. musculus, the genetic evolutionary tree can be divided into three branches. Conclusion Nucleotide polymorphism from M. musculus is rich. The high genetic diversity was indicated within M. musculus, which had a connection with geographical distribution.
HU Shuang-shuang, LU Yong-chang, WU Bing-yao, YANG Qing-gui, CAI Jia, HE Jiang, SUN Li-xin
. Genetic polymorphisms and phylogenetic relationships of Mus musculus based on sequences of mitochondrial DNA D-loop[J]. Chinese Journal of Vector Biology and Control, 2017
, 28(4)
: 332
-335
.
DOI: 10.11853/j.issn.1003.8280.2017.04.006
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