Identification of 49 common fly species in Calliphoridae based on DNA barcodes

QIU De-yi, LIAO Jun-lei, WEI Xiao-ya, YUE Qiao-yun

doi:10.11853/j.issn.1003.8280.2017.04.005

Chinese Journal of Vector Biology and Control >

2017 , Vol. 28 >Issue 4: 322 - 331

DOI: https://doi.org/10.11853/j.issn.1003.8280.2017.04.005

Identification of 49 common fly species in Calliphoridae based on DNA barcodes

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1 Zhongshan Entry-Exit Inspection and Quarantine Technology Center, Zhongshan 528403, Guangdong Province, China;
2 Life College of Sun Yat-sen University

Received date: 2017-04-17

Online published: 2017-08-20

Supported by

Supported by the National Quality Infrastructure Project (No. 2016YFF0203205), Administration of Quality Supervision, Inspection and Quarantine Support Program of China (No. 2015IK067, 2015IK069), and Guangdong Province International Cooperation Project (No. 2015A050502009)

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Abstract

Objective To establish the DNA barcoding identification techniques for 49 common fly species in Calliphoridae, and provide references data for the identification of these species with DNA barcodes, as to solve the difficulties in the accurate identification of the females, individuals with lost morphological features, and larvae of these species. Methods Totally 166 individuals in Calliphoridae were identified with the morphological characters which were collected from different areas in China during 2012 to 2015, DNA barcodes of the males and females were obtained, an N-J tree was constructed in combination with published data. Consistency of the identification results by morphology and DNA barcodes were tested as well. Results All the 166 sequences of these 49 species were submitted to the public data base, DNA barcodes sequences of 28 species were obtained for the first time. The differences of the intra-species was less than 1%, and the inter-species DNA barcode differences increasing with the morphology differences, and the identification results based on DNA barcodes were consistent with that based on morphology. Conclusion The application of DNA barcodes were strong supplemental to the morphology identification of the species, especially to the incomplete, non-adult and female individuals, and can increase accuracy of identification, and it is very important for the rapid and accurate species identification of the medical vectors at the ports.

Key words： Calliphoridae; DNA barcodes; Species identification

Cite this article

QIU De-yi, LIAO Jun-lei, WEI Xiao-ya, YUE Qiao-yun . Identification of 49 common fly species in Calliphoridae based on DNA barcodes[J]. Chinese Journal of Vector Biology and Control, 2017 , 28(4) : 322 -331 . DOI: 10.11853/j.issn.1003.8280.2017.04.005

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