Objective To investigate the effect of larval nutritional regimes on the body size, physiological condition and sugar feeding behavior of Culex pipiens pallens adults. Methods Culex pipiens pallens larvae, which were collected from water pools in the urban residential areas of Hangzhou city in August, 2014, were reared with three diet regimes, and the wing length and body weight of newly emerged adults, as well as the contents of glycogen, lipid and protein, were determined. Newly emerged adults from different larval nutritional regimes were provided with glucose solution as sugar source, and the temporal change of proportions of sugar-fed mosquitoes was determined. Results The wing length of newly emerged female and male mosquitoes from different groups was 4.26-3.57 mm and 3.53-2.99 mm respectively, their body weight was 3.16-1.53 mg and 1.95-1.12 mg respectively, the differences were statistically significant between these larval nutritional regimes (P<0.05). The content of sugar from individual newly emerged female and male mosquitoes was 41.76-19.90 μg and 39.62-14.66 μg respectively; the content of lipid was 48.23-17.48 μg and 52.36-9.42 μg respectively; the content of protein was 59.82-24.85 μg and 68.07-34.23 μg respectively; the energy reserves of newly emerged female and male Cx. pipiens pallens were 3.70-1.58 J and 3.68-1.03 J, respectively. The proportion of sugar-fed newly emerged mosquitoes from rich larval nutritional regimes was highest among the groups, which was 70.80% for females and 53.30% for males, and the values continuously increased since adults emerged. Conclusion The wing length and body weight, as well as the content of sugar, lipid and protein, were different in newly emerged female and male Cx. pipiens pallens from different nutritional regimes; the abundance of larval food could significantly affect the body size, physiological condition of Cx. pipiens pallens adults and their tendency to sugar sources.
YU Bao-ting, HU Yin, LIAN Liu-qing, SONG Xiao-gang, DING Yan-mei, MO Jian-chu
. Effect of larval nutritional regimes on the physiological condition and sugar feeding behavior of Culex pipiens pallens[J]. Chinese Journal of Vector Biology and Control, 2017
, 28(4)
: 314
-317
.
DOI: 10.11853/j.issn.1003.8280.2017.04.003
[1] Foster WA. Mosquito sugar feeding and reproductive energetics[J]. Annu Rev Entomol,1995,40:443-474.
[2] Hancock RG, Foster WA. Larval and adult nutrition effects on blood/nectar choice of Culex nigripalpus mosquitoes[J]. Med Vet Entomol,1997,11(2):112-122.
[3] Ma-ga H, Dabiré RK, Lehmann T, et al. Variation in energy reserves and role of body size in the mating system of Anopheles gambiae[J]. J Vector Ecol,2012,37(2):289-297.
[4] Telang A, Li Y, Noriega FG, et al. Effects of larval nutrition on the endocrinology of mosquito egg development[J]. J Exp Biol, 2006,209(4):645-655.
[5] Kassim NFA, Webb CE, Russell RC. The importance of males:larval diet and adult sugar feeding influences reproduction in Culex molestus[J]. J Am Mosq Control Assoc,2012,28(4):312-316.
[6] Vaidyanathan R,Fleisher AE,Minnick SL,et al. Nutritional stress affects mosquito survival and vector competence for West Nile virus[J]. Vector Borne Zoonotic Dis,2008,8(6):727-732.
[7] Takken W,Smallegange RC, Vigneau AJ, et al. Larval nutrition differentially affects adult fitness and Plasmodium development in the malaria vectors Anopheles gambiae and Anopheles stephensi[J]. Parasit Vectors,2013,6(1):345.
[8] Impoinvil DE, Kongere JO, Foster WA, et al. Feeding and survival of the malaria vector Anopheles gambiae on plants growing in Kenya[J]. Med Vet Entomol,2004,18(2):108-115.
[9] Manda H,Gouagna LC,Foster WA,et al. Effect of discriminative plant-sugar feeding on the survival and fecundity of Anopheles gambiae[J]. Malaria J,2007,6(1):113.
[10] Foster WA,Takken W. Nectar-related vs. human-related volatiles:behavioural response and choice by female and male Anopheles gambiae (Diptera:Culicidae) between emergence and first feeding[J]. Bull Entomol Res,2004,94(2):145-157.
[11] Shin SM, Akram W, Lee JJ. Effect of body size on energy reserves in Culex pipiens pallens females (Diptera:Culicidae)[J]. Entomol Res,2012,42(3):163-167.
[12] Ma-ga H, Niang A, Sawadogo SP, et al. Role of nutritional reserves and body size in Anopheles gambiae males mating success[J]. Acta Trop,2014,132 Suppl:S102-107.
[13] Yahouédo GA, Djogbénou L, Sa-zonou J, et al. Effect of three larval diets on larval development and male sexual performance of Anopheles gambiae s. s[J]. Acta Trop,2014,132 Suppl:S96-101.
[14] Okanda FM, Dao A, Njiru BN, et al. Behavioural determinants of gene flow in malaria vector populations:Anopheles gambiae males select large females as mates[J]. Malaria J,2002,1(1):10.
[15] Briegel H, Knusel I, Timmermann SE. Aedes aegypti:size, reserves, survival, and flight potential[J]. J Vector Ecol, 2001,26(1):21-31.
[16] Telang A, Wells MA. The effect of larval and adult nutrition on successful autogenous egg production by a mosquito[J]. J Insect Physiol,2004,50(7):677-685.
