Objective To survey of ectoparasite and Yersinia pestis from Vormela peregusna in the China-Kazakhstan border. Methods The ectoparasites from V. peregusna were collected and identified by morphology. Also Y. pestis were tested in the V. peregusna samples during the 2007-2016 in the China-Kazakhstan border. Results A total 615 ticks (Haemaphysalis erinacei turanica, Ixodes redikorzevi, Rhipicephalus sanguineus and Ornithodors tartakovskyi)and 284 fleas (Xenopsylla minax, Echidnophaga oschanini, Nopsyllus laeviceps laeviceps and Pulex irritans)were collected from 14 V. peregusna, which belonged to 4 species, 4 genera, 2 families for both ectoparasites. In this study, H. erinacei turanica was predominant species in ticks and O. tartakovskyi was first collected. A new flea species P. irritans also was collected from the V. peregusna. Meanwhile, there was no positive for Y. pestis in 14 V. peregusna samples. Conclusion Diversity and large numbers of ectoparasites were found in V. peregusna. There is significant concern over plague epidemiology, and the infection situation need to be further monitored.