Objective To analyze the reasons for antigen diversity of Anaplasma phagocytophilum grown in different cells. Methods The P44 transcripts were analyzed using nested RT-PCR, and the P44 proteins were produced as recombinant protein in vitro by insect cell-free protein synthesis system. Results We detected and characterized P44 transcript variants of A. phagocytophilum from THP-1 and HL60 cells. And our study demonstrated the presence of specific antibodies against the 3 type of rP44 proteins that prevailed in infected THP-1 and HL60 cells, probably being suitable as protein antigens for sero-diagnosis of human granulocytic anaplasmosis. Conclusion We demonstrated antigenic diversity of a variety of A. phagocytophilum grown in different cells.
Gaowa, YIN Xu-hong, GUO Sheng-chun, Wulantuya, CAO Min-zhi
. Analysis of antigenic diversity of Anaplasma phagocytophilum grown in different cells[J]. Chinese Journal of Vector Biology and Control, 2017
, 28(3)
: 255
-257
.
DOI: 10.11853/j.issn.1003.8280.2017.03.015
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