Objective To identify the transmission capacity of Babesia microti through Haemaphysalis japonica. Methods The larvae, nymphs, and adults of H. japonica were fed on BALB/c mice infected with B. microti. The engorged ticks were collected and maintained to the next stage. Ticks of the next stage or the offspring larvae were detected by nested PCR. NOD/SCID mice were then infested by adult ticks molting from nymphs that ingested the blood of infective mice, and the blood samples of NOD/SCID mice were then analyzed by PCR. Results None of the nymphs developed from larvae that fed on positive mice tested positive. Among adult ticks developed from nymphs that were exposed to positive mice, 40% of the females and none of the males tested positive. Among the 12 mice infested by infective adults, B. microti was detected in two NOD/SCID. None of the offspring larvae developed from adult ticks that fed on infected mice tested positive. Conclusion Experimental transstadial transmission of H. japonica for B. microti was proved in the nymph to adult route, but neither in the larvae to nymph routes nor transovarial transmission.
WU Jia-tong, ZHANG Yi, ZHU Dan, LI Lan-hua
. Experimental transmission of Babesia microti by Haemaphysalis japonica[J]. Chinese Journal of Vector Biology and Control, 2017
, 28(3)
: 248
-250
.
DOI: 10.11853/j.issn.1003.8280.2017.03.013
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