ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Epidemiological investigation and pathogen identification in three cases of brucellosis from Suizhou city, Hubei province in 2016
Received date: 2016-12-09
Online published: 2017-04-20
Objective Three suspected brucellosis cases were investigated in Suizhou city of Hubei province, and three suspected Brucella strains were identified. Methods Field investigation was applied to finish Brucella epidemiological survey, used Brucella conventional appraisal classification method and the phage cracking test for testing. BCSP31-PCR method (Based on Brucella genus specific gene BCSP31 target gene detection method), AMOS-PCR (based on electrophoresis banding identify B. abortus biovar 1, 2, 4 of Brucella, B. melitensis, B. ovis and B. suis biovar 1) and Real-time PCR, three molecular classification method were compared. Results Three patients were infected by close contact with sick animals and their meat, conventional identification and a variety of polymerase chain reaction (PCR) had the same results, B. melitensis biovar 3. Conclusion Brucella melitensis biovar 3 was identified as the pathogen of the three cases from Suizhou city and cases were mainly caused by direct contact with sick animals and their meat.
Key words： Brucella; Individual survey; Polymerase chain reaction; Identification
ZHAO Zhong-zhi, NIE Dan-wen, HAN Xiu-rui, LIU Zhi-guo, PIAO Dong-ri, JIANG Hai, TIAN Guo-zhong, ZHAO Hong-yan, CUI Bu-yun . Epidemiological investigation and pathogen identification in three cases of brucellosis from Suizhou city, Hubei province in 2016[J]. Chinese Journal of Vector Biology and Control, 2017 , 28(2) : 163 -165 . DOI: 10.11853/j.issn.1003.8280.2017.02.017
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