First detection of Wolbachia in Culex modestus at Alataw port, China-Kazakhstan border

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  • 1 Alashankou Entry-Exit Inspection and Quarantine Bureau, Alashankou 833418, Xinjiang Uygur Autonomous Region, China;
    2 College of Animal Science and Technology, Shihezi University

Received date: 2016-11-21

  Online published: 2017-04-20

Supported by

Supported by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China Scientific Research Subject(No. 2016IK264)

Abstract

Objective To investigate the infection and genotype of Wolbachia from Culex modestus at Alataw port, China-Kazakhstan border. Methods Sweeping Netting and Light Trapping were utilized to collect adult mosquitoes at the Yiqu, Erqu and Aibi Lake, wsp genes of Wolbachia were detected by polymerase chain reaction (PCR), the sequence analysis by Blast and phylogenetic analysis by Mega 6.0. Results The positive rate of wsp gene of Wolbachia was 3.33% (2/60) at Alataw port. The wsp haplotype sequences were divided in Supergroup A (wAlbA) and B (wAlbB) by phylogenetic analysis. Conclusion The wAlbA and wAlbB Wolbachia exists in Cx. modestusat Alataw China-Kazakhstan port.

Cite this article

YIN Xiao-ping, WANG An-dong, TIAN Yan-he, BA Te, ZHANG Jiang-guo, LIANG Zhen . First detection of Wolbachia in Culex modestus at Alataw port, China-Kazakhstan border[J]. Chinese Journal of Vector Biology and Control, 2017 , 28(2) : 117 -119 . DOI: 10.11853/j.issn.1003.8280.2017.02.005

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