Objective To test the resistance to pyriproxyfen in dengue vectors Aedes aegypti and Ae. albopictus and observe the mortality pattern of mosquitoes at various concentrations in Jinghong city, Yunnan province, so as to provide evidence for dengue vectors control and resistance management. Methods Aedes larvae were collected from Dengue epidemic areas in Jinghong from October to November, 2015. WHO recommended methods for insect growth regulators was carried out on the 3^rd instar larvae collected. The resistance ratio (RR) was calculated by comparing half inhibition of emergence concentration (IE₅₀) of the tested wild population with the susceptible reference strain. Additionally, the mortality pattern of dengue vectors exposed to pyriproxyfen was analyzed by observing daily stage-specific mortality at various concentrations. Results The IE₅₀s of Ae. aegypti and Ae. albopictus in Jinghong city were 0.024 47 (0.022 09-0.027 07) and 0.135 20 (0.117 10-0.156 60) μg/L. There was 13.44-fold resistance in Ae. albopictus and 0.85-fold resistance in Ae. aegypti to pyriproxyfen compared with the susceptible strains respectively. Aedes albopictus was also 5.53-fold more tolerant to pyriproxyfen than Ae. aegypti in Jinghong city. For the dead individuals, most of them died at 4-6 d after exposure to pyriproxyfen, and the peak of death for susceptible Ae. aegypti and Ae. albopictus were at 4-5 d and 4 d respectively. A wider range of death peak was observed for Jinghong population of Ae. aegypti and Ae. albopictus, which was at 4-6 d. At low concentrations, most of the dead were deformed adults and mosquitoes not completely separated from the pupae. For the susceptible reference strain, dead pupae began to occur steadily when the mortality was over 60%, and the proportion increased as the concentration went up. However, the mortality of pupae was fluctuating in Jinghong population. Conclusion In Jinghong city, Ae. aegypti larvae was susceptible to pyriproxyfen. Considering this species is preferentially found in urban areas, pyriproxyfen is recommended to use in urban areas. Although Ae. albopictus larvae have developed moderate resistance to pyriproxyfen, this larvicide still can be used in alternatery with other insecticides to delay the resistance development. It proves that pyriproxyfen is highly efficient for mosquito control and resistance management in Jinghong city. Periodic resistance monitoring must be conducted to learn the variations of resistance level timely.