ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Identification of rodent species in Zhejiang port using cytochrome C oxidase subunitⅠ and 16S rRNA gene and analysis of sequence characteristics
Received date: 2016-10-24
Online published: 2017-04-20
Supported by
Supported by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China Scientific Research Subject(No. 2015IK303)
Objective To identify rodent species in Zhejiang port and analyze the sequence characteristics. Methods The livers were obtained from rodents captured during April to October in 2015. The cytochrome C oxidase subunitⅠ(COⅠ) and 16S rRNA gene sequences of rodents were amplified. The species were confirmed and the phylogenetic trees were constructed. Results There were two species, Rattus norvegicus and Mus musculus in Zhejiang port. The mutation rate of COⅠgene was greater than that of 16S rRNA gene. Most of the mutation was transition and synonymous mutation. Conclusion Both COⅠ gene and 16S rRNA gene, as molecular markers, could be applied to identify rodent species.
Key words： Rodents; Phylogenetic trees; Identification
QI Run-zi, WANG Dong, PU Li-ya, ZHAO Xin, GAO Xue-meng, MA Xiang, YU Bin, ZHANG Xiao-long . Identification of rodent species in Zhejiang port using cytochrome C oxidase subunitⅠ and 16S rRNA gene and analysis of sequence characteristics[J]. Chinese Journal of Vector Biology and Control, 2017 , 28(2) : 104 -107 . DOI: 10.11853/j.issn.1003.8280.2017.02.002
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