ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Identification and distribution of Apodemus species with DNA barcoding in China
Received date: 2016-10-31
Online published: 2017-04-20
Supported by
Supported by the National Science and Technology Major Project of China (No. 2012ZX10004-219)
Objective To investigate the taxonomy and distribution of Apodemus species in China, and to provide supportive data for prevention and control of rodent borne disease. Methods DNA barcoding was employed to identify Apodemus samples collected from 22 provincial areas in China. Genomic DNA was extracted from tissue samples for PCR amplification of the mitochondrial cytochrome C oxidase subunitⅠ (COⅠ) gene, followed by sequencing. The obtained sequences were compared with the related sequences in GenBank. Then a phylogenetic tree was constructed. Results COⅠ gene fragment were amplified from 119 Apodemus samples, and they were sorted into eight branches through phylogenetic reconstruction. Compared with the data in the GenBank, the collected samples were identified as eight independent species: Ap. agrarius, Ap. chevrieri, Ap. peninsulae, Ap. latronum, Ap. ilex, Ap. draco, Ap. uralensis and an undefined species. Conclusion The accurate distribution of Apodemus species in China can be determined by effective identification of samples through DNA barcoding. In addition, the results revised the Apodemus species distribution based on the morphological identification.
Key words： DNA barcoding; Apodemus; Species identification; Distribution
LIU Rong-rong, GE De-yan, LU Liang, XIA Lin, LIU Qi-yong, YANG Qi-sen . Identification and distribution of Apodemus species with DNA barcoding in China[J]. Chinese Journal of Vector Biology and Control, 2017 , 28(2) : 97 -103 . DOI: 10.11853/j.issn.1003.8280.2017.02.001
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