ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Frequency of alleles associated with insecticide resistance in a field housefly population from Beihai city, Guangxi Zhuang Autonomous Region
Received date: 2016-09-07
Online published: 2017-02-20
Supported by
Supported by the National Science and Technology Major Project of China（No. 2012ZX10004219）
Objective To understand the current status of insecticide resistance of housefly in Beihai city of Guangxi Zhuang Autonomous Region by molecular tools. Methods The genotypes of three genes(CYP6D1, Vssc and ace) in a field population of housefly from Beihai city were detected by polymerase chain reaction-restriction fragment length polymorphism analyses, and corresponding frequencies for each genotype were recorded. Results In 63 houseflies collected from Beihai city, no homozygote for CYP6D1 resistant allele was detected, while the frequencies of susceptible homozygotes and heterozygotes were 96.83%(61/63) and 3.17%(2/63) respectively. No 1014H mutation of Vssc was observed in these samples, but 1014F mutation was present in heterozygotes in a low frequency of 6.35%(4/63). The frequency of 1014L/L(susceptible) homozygote was very high(59/63, 93.65%). In 38 individuals, we observed two ace genotypes, namely 342G/V and 342A/V, in frequencies of 5.26% and 94.74% respectively. Notably, the frequency of resistant alleles(342A and 342V) reached 97.37%. Conclusion The frequencies of resistant alleles of both CYP6D1 (1.59%) and Vssc(3.18%) were low, suggesting that pyrethroids can be used for housefly control in this area. In contrast, extremely high frequencies of resistant alleles of ace were detected in housefly samples from Beihai city, indicating that failure may occur if using organophosphates and carbamates for control of house flies in the sampling region.
DING Yan, YANG Chan, LI Mei, FENG Xiang-yang, QIU Xing-hui . Frequency of alleles associated with insecticide resistance in a field housefly population from Beihai city, Guangxi Zhuang Autonomous Region[J]. Chinese Journal of Vector Biology and Control, 2017 , 28(1) : 12 -15 . DOI: 10.11853/j.issn.1003.8280.2017.01.004
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