ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Detection and phylogenetic analysis of Wolbachia in Aedes albopictus from different geographical populations
Received date: 2016-09-27
Online published: 2017-02-20
Supported by
Supported by the National Natural Science Foundation of China（No. 81401693, 81572028）and the Student's Platform for Innovation and Entrepreneurship Training Program（No. 201510439010）
Objective Investigate Wolbachia infection and diversity in Aedes albopictus from different populations of China,in order to provide baseline data for the prevention and control of mosquito-borne diseases. Methods Choosing Wolbachia surface protein gene(wsp) as reference. All wsp DNA sequences obtained through PCR and sequenced were used for comparison with those sequences downloaded from GenBank, to determine the states and types of Wolbachia that infected by Ae. albopictus. Results Eleven populations were infected with Wolbachia, which including wAlbA subgroup of A group, and wPip, wAlbB subgroup of B group. Additionally, samples from Taian and Yulin were found coinfected with A and B group. These results were further supported by phylogenetic analysis, which demonstrated that all wsp sequences were clustered into two clades, and two subclades in B group were corresponding with wPip and wAlbB. Conclusion Aedes albopictus of our country were mainly infected with wAlbA and wAlbB subgroups, which with high infect rate.
Key words： Mosquito-borne disease; Wolbachia; Aedes albopictus; wsp gene
ZHANG Rui-ling, YAO Guang-qin, PAN Xiao-qian, ZHAO Ai-hua, MA De-zhen, ZHANG Zhong . Detection and phylogenetic analysis of Wolbachia in Aedes albopictus from different geographical populations[J]. Chinese Journal of Vector Biology and Control, 2017 , 28(1) : 9 -11 . DOI: 10.11853/j.issn.1003.8280.2017.01.003
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