ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Francisella tularensis was detected in Haemaphysalis longicornis from Liaoning province
Received date: 2016-07-08
Online published: 2016-12-20
Supported by
Supported by the National Natural Science Foundation of China(No. 81473032)
Objective To detect the infection of Francisella and Francisella-like endosymbiont(FLEs)in Haemaphysalis longicornis from Liaoning province. Methods In July 2013, 357 H. longicornis collected in Liaoning province were divided into 38 groups, and cultured for Francisella tularensis. Detection of Francisella and FLEs was performed using nested polymerase chain reaction(PCR)assays based on the amplification of 16S rRNA. The PCR products amplified with target fragment were sequenced, and then their nucleotide sequences obtained were aligned and performed phylogenetic analysis with published sequences of Francisella and FLEs in GenBank. Results In the sequenced 13 groups of H. longicornis, 5 groups belonged to F. tularensis, other 8 to FLEs. It was calculated that the minimum carrying rate with F. tularensis in H. longicornis from Helan town was 2.12%. The phylogenetic analysis indicated that 5 groups were assigned to a clade of F. tularensis, and the rest 8 groups to main clade with FLEs. Conclusion The current work is the first detection of the infection of F. tularensi and FLEs in H. longicornis from Liaoning province, which serves a warning to the health department within the region to do further risk assessment on the emergence of tularemia.
WANG Yan-hua, MAO Ling-ling, PENG Yao, SUN Ying-wei, WANG Zi-jiang, ZHANG Jia-yong, ZHANG Ji-bo, TIAN Jiang . Francisella tularensis was detected in Haemaphysalis longicornis from Liaoning province[J]. Chinese Journal of Vector Biology and Control, 2016 , 27(6) : 529 -532 . DOI: 10.11853/j.issn.1003.8280.2016.06.001
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