Objective To comprehend the epidemiological trend of human Brucellosis in Qinghai province in 2009-2014. Methods Analyzed the investigation results of human Brucellosis in 2 cities, 2 districts and 18 counties in Qinghai province during 2009 to 2014. Results In 2009-2014, we investigated 24 831 humans, the annual infection rate were, in order, 5.30%, 6.43%, 5.76%, 2.78%, 3.09%, and 3.95%. The annual prevalence rate was, in order, 2.74%, 2.35%, 2.25%, 0.87%, 1.39%, and 2.21%. The average positive rate of RBPT was 4.16%(1 032/24 831), SAT was 1.71%(425/24 831), Coombs was 2.04%(21/1 027). Newly diagnosed cases were 316, each year's annual incidence was rising, results were statistically significant(χ²=66.505, P < 0.01). The cases were mainly concentrated in Henan, Tianjun, Gonghe, Jiuzhi, Dari county and other breeding and slaughtering animals based grassland farming counties. The number of cases were, in order, pastoral region(238), towns(43), agriculture region(31), farming-pastoral region(4). Conclusion Prevalence of human Brucellosis in Qinghai province is active with spreading tendency.