ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
DNA barcoding and morphology identification of Pollenia rudis－a non-recorded fly species in China
Received date: 2016-04-05
Online published: 2016-08-20
Supported by
Supported by the National Science and Technology Support Program(No. 2012BAK11B05), Guangdong Province Science and Technology Program (No. 2015A050502009), and the Administration of Quality Supervision, Inspection and Quarantine Program of China(No. 2015IK067, 2015IK069)
Objective To identify the female fly intercepted from an entry ship rapidly and accurately with ‘Barcoding and Morphology’ (B & M)techniques. Methods Identifying the female fly which were intercepted at Zhongshan Xiaolan port on an entry ship from Ireland with waste paper on March 26 of 2015 by morphology and DNA barcoding, and comparing with its similar species Pollenia pediculate. Results The intercepted fly was P. rudis ♀. The differences between P. rudis and P. pediculate are subtle in morphology, but with 32 nucleotides differences in the 658 bp DNA barcoding fragment. The difference between these two species is up to 4.86%, far above 2%, the normal accepted species boundary with DNA barcoding. Conclusion The ‘B & M’ technique is a good tool to identify the female flies, especially the alien ones when no references are available.
WEI Xiao-ya, QIU De-yi, YUE Qiao-yun, LIU De-xing . DNA barcoding and morphology identification of Pollenia rudis－a non-recorded fly species in China[J]. Chinese Journal of Vector Biology and Control, 2016 , 27(4) : 326 -329 . DOI: 10.11853/j.issn.1003.8280.2016.04.003
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