ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
The role of cell membrane cholesterol in Japanese encephalitis virus and enterovirus 71 infection of human neuronal cells
Received date: 2016-02-19
Online published: 2016-06-20
Objective To investigate the role of cell membrane cholesterol in Japanese encephalitis virus (JEV) and enterovirus 71 (EV71) infection of human neuronal cells.Methods CCK-8 cell counting assay was conducted to detect the cytotoxicity of cholesterol depletion agent, ethyl-β-cyclodextrin (MβCD) on SH-SY5Y cells. The impact of MβCD on JEV and EV71 mRNA expression was validated by quantitative real-time PCR (qRT-PCR) after SH-SY5Y was pretreated by MβCD. Then MβCD was added at different stages of virus infection and detected the expression level of JEV and EV71 mRNA. Results The highest working concentration of MβCD was 5 mmol/L.Data of qRT-PCR showed that the expression of JEV and EV71 mRNA was inhibited by pretreatment of MβCD (P < 0.05), while addition of MβCD at 60 min after the incubation of EV71 had little effect (P > 0.05).Conclusion Cell membrane cholesterol plays an important role in JEV and EV71 infection of human neuronal cells, but acts at different stages.
CHEN Sheng-lin, ZHU Nai-wei, ZHU Yong-zhe, XU Qing-qiang, PENG Hao-ran, QI Zhong-tian . The role of cell membrane cholesterol in Japanese encephalitis virus and enterovirus 71 infection of human neuronal cells[J]. Chinese Journal of Vector Biology and Control, 2016 , 27(3) : 253 -256 . DOI: 10.11853/j.issn.1003.8280.2016.03.010
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