ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Investigation of the Bartonella infection in wild rodent populations in Ningxia Hui Autonomous Region, China
Received date: 2016-03-02
Online published: 2016-06-20
Supported by
Supported by the National Science and Technology Major Project of China (No. 2012ZX10004-219)
Objective To investigate the Bartonella infections in wild rodent populations in Ningxia Hui Autonomous Region, China, and provide support for the prevention and control of Bartonella infections in humans. Methods Rodents were collected from different regions and habitats by the night trapping method. The livers and spleens were sampled using aseptic technique and the specific primers of Bartonella spp. were used to amplify the gltA gene fragment using PCR. Then the ftsZ, rpoB, and 16S rRNA genes of the positive samples were amplified and positive amplicons were sequenced. The nucleic acid sequences were submitted to the GenBank. The multiple sequence alignment of the sequences was Mega 6.06 software. The neighbor-joining tree was inferred from gltA similarity data with Mega 6.06 software. Results Five sampling sites were selected from Helan Mountain, Yinchuan, and Zhongwei city as well as Guyuan. The sampling sites were categorized into four habitats including grassland, shrub, farmland and forest, from which 233 samples were collected. Bartonella was cultivated in 51 samples giving a positive rate of 21.79% (51/234). A total of 13 genera, 20 species of rodents were captured. The Bartonella was isolated from 9 species, 7 genera of rodents. The rates of isolation among different species were: 45.45% (5/11) in Spermophilus alaschanicus, 33.33% (15/45) in Apodemus agrarius, 33.33%(11/33) in Ap. peninsulae, 25.00% (5/20) in Dipus sagitta, 20.83% (5/24) in Meriones meridianus, 17.86% (5/28) in Cricetulus longicaudatus, 14.29% (1/7) in Meriones unguiculatus, 13.64% (3/22) in Cricetidae and 11.11% (1/9) in Allactaga sibirica. Spermophilus alaschanicus infection rate was highest. The Bartonella isolates were found to be most similar to B. grahamii, B. elizabethae, B. jaculi, B. japonica and B. taylorii. Conclusion This report provides evidence of Bartonella infections in wild rodent populations in Ningxia region. The high prevalence of Bartonella spp. exists in various rodents and habitats. Phylogenetic analysis showed high diversity in the Bartonella isolates comprising of the pathogenic species which could pose risk of human infections.
Key words： Bartonella; Rodents; Phylogenetic analysis
JIANG Ya-yun, LU Liang, SONG Xiu-ping, YUE Yu-juan, WANG Jun, LIU Qi-yong, LI Jin-chun, LI Dong-mei . Investigation of the Bartonella infection in wild rodent populations in Ningxia Hui Autonomous Region, China[J]. Chinese Journal of Vector Biology and Control, 2016 , 27(3) : 235 -240 . DOI: 10.11853/j.issn.1003.8280.2016.03.006
[1] Li DM, Hou Y, Song XP, et al. High prevalence and genetic heterogeneity of rodent-borne Bartonella species on Heixiazi Island,China[J]. Appl Environ Microbiol,2015,81(23):7981-7992.
[2] 白瑛,Kosoy MY,Maupin GO,等. 首次证实巴尔通体在我国云南鼠群中流行[J]. 中国人兽共患病杂志,2002,18(3):5-9.
[3] 栗冬梅,俞东征,刘起勇,等. 云南省不同环境鼠形动物巴尔通体感染情况的研究[J]. 中华流行病学杂志,2004,25(11):934-937.
[4] 叶曦,李国伟,姚美琳,等. 福建省沿海地区鼠形动物巴尔通体感染状况调查及基因类型研究[J]. 中华流行病学杂志, 2009,30(10):989-992.
[5] 林国华,颜翠兰. 龙海市鼠形动物巴尔通体感染调查[J]. 中国人兽共患病学报,2010,26(10):989.
[6] 宋秀平,刘起勇,鲁亮,等. 海南省小型兽类巴尔通体的分离培养和序列分析[J]. 中国媒介生物学及控制杂志,2010,21(2):131-133.
[7] 左双燕. 我国黑龙江林区鼠型动物巴尔通体感染调查与分离鉴定[D]. 长沙:中南大学,2012.
[8] 凌锋,丁丰,龚震宇,等. 浙江省啮齿动物巴尔通体感染状况调查[J]. 中国媒介生物学及控制杂志,2014,25(1):24-27.
[9] Kosoy MY, Regnery RL, Tzianabos T, et al. Distribution, diversity, and host specificity of Bartonella in rodents from the Southeastern United States [J]. Am J Trop Med Hyg,1997,57(5):578-588.
[10] 张久存,李莺. 7例猫抓病误诊分析[J]. 宁夏医学院学报, 2008,30(5):679-680.
[11] Norman AF, Regnery R, Jameson P, et al. Differentiation of Bartonella-like isolates at the species level by PCR-restriction fragment length polymorphism in the citrate synthase gene[J]. J Clin Microbiol,1995,33(7):1797-1803.
[12] Zeaiter Z, Liang ZX, Raoult D. Genetic classification and differentiation of Bartonella species based on comparison of partial ftsZ gene sequences[J]. J Clin Microbiol,2002,40(10):3641-3647.
[13] Renesto P, Gouvernet J, Drancourt M, et al. Use of rpoB gene analysis for detection and identification of Bartonella species[J]. J Clin Microbiol,2001,39(2):430-437.
[14] 栗冬梅,苗志刚,宋秀平,等. 巴尔通体液体培养条件简化及生长曲线观察[J]. 微生物学通报,2012,39(11):1695-1702.
[15] Tamura K, Stecher G, Peterson D, et al. MEGA6: molecular evolutionary genetics analysis version 6.0[J]. Mol Biol Evol, 2013,30(12):2725-2729.
[16] 栗冬梅,刘起勇,宋秀平,等. 中国家猫克氏巴尔通体分离株生物学及分子特征分析[J]. 微生物学报,2009,49(4):429-437.
[17] 杨发莲,白鹤鸣,杨慧,等. 巴尔通体分离培养及生物学特性研究[J]. 中国热带医学,2007,7(11):2001-2004.
[18] 黄起翡,李平航,危玲. 探讨基于不同基因设计的巴尔通体特异引物[J]. 中国媒介生物学及控制杂志,2014,25(3):284-288.
[19] Breitschwerdt EB, Kordick DL. Bartonella infection in animals: carriership, reservoir potential, pathogenicity, and zoonotic potential for human infection[J]. Clin Microbiol Rev,2000,13(3):428-438.
[20] Oksi J, Rantala S, Kilpinen S, et al. Cat scratch disease caused by Bartonella grahamii in an immunocompromised patient[J]. J Clin Microbiol, 2013, 51(8):2781-2784.
/
| 〈 |
|
〉 |