ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Resistance of Aedes albopictus in Chaozhou city, China to commonly used insecticides
Received date: 2016-04-27
Online published: 2016-06-20
Supported by
Supported by the National Science and Technology Major Project of China (No. 2012ZX10004-219) and the National Science and Technology Infrastructure Program (No. 2014BAI13B05)
Objective To investigate the resistance of larvae and adults of Aedes albopictus to commonly used insecticides in Chaozhou, Guangdong province in China during the dengue outbreak in 2015, providing guidance in the control of dengue vector mosquitoes. Methods Along with the efforts to control the outbreak, larval mosquitoes were collected from the epidemic areas, recording the number and instar of larvae, as well as the longitude and latitude of collecting sites. According to the National Scheme of Pesticide Resistance Surveillance for Major Vectors (Trial) PartⅡ Aedes aegypti and Aedes albopictus, WHO recommended methods-bioassays were performed on the collected Ae. albopictus. With reference to the S-lab (a laboratory strain from National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention), insecticides resistance was tested with exposure tubes on adults and dipping method on larvae. Results The median lethal concentration (LC50) of the larvae against deltamethrin, permethrin, beta-cypermethrin and temephos were 0.092 8 mg/L (RR=232.0), 2.047 5 mg/L (RR=1 023.8), 0.055 2 mg/L (RR=61.3) and 0.007 3 mg/L (RR=4.6) respectively. Adult mosquitoes were tested against 3% permethrin for one hour, and only 66.67% died 24 h later, showing resistance to permethrin. When exposed to 1.8% cyhalothrin, 0.1% deltamethrin, 3% beta-cypermethrin and 0.44% malathion for 1 h respectively, the mortality 24 h later were 86.96%, 95.12%, 82.50%, and 91.43%, indicating decreased susceptibility and likely resistance/tolerance to the insecticides. However, the tested adults showed high susceptibility to 0.18% fenitrothion, 0.6% dichlorvos and 0.06% propoxur with mortality 100% (1 h exposure and 24 h recovery). Conclusion The larvae of Ae. albopictus in Chaozhou city have developed resistance to the tested insecticides, especially to pyrethroids. Adult mosquitoes showed high resistance to permethrin, but further researches are needed to confirm the resistance to deltamethrin, cyhalothrin, beta-cypermethrin and malathion. In practice, insecticides resistance surveillance should be highlighted to enhance mosquito control efficacy.
Key words： Chaozhou city; Dengue; Aedes albopictus; Insecticide resistance; Bioassay
WANG Yi-guan, SHI Can-nan, LIN Guo-song, LIU Qi-yong, LIU Shao-qun, DU Guang-wen, SONG Tie, CAI Zhong-xian, MENG Feng-xia . Resistance of Aedes albopictus in Chaozhou city, China to commonly used insecticides[J]. Chinese Journal of Vector Biology and Control, 2016 , 27(3) : 228 -231 . DOI: 10.11853/j.issn.1003.8280.2016.03.004
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