ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
The application of tandem repeat in genetic typing Yersinia pestis of Hebei province
Received date: 2015-12-21
Online published: 2016-04-20
Supported by
Supported by the Youth Science and Technology Project of Hebei Provincial Health Department (No. ZL20140119)
Objective To study the genotype of Yersinia pestis in Hebei plague foci by variable number of tandem repeat (VNTR). Methods Primers were designed according to the confirmed 14＋12 VNTR, to genotype the 116 Y. pestis DNA of Hebei province. Results All of the strains showed one genetype, but they were different from CO92 and EV. Conclusion There is only one genetype of plague, indicating a genetic stability in Y. pestis in Hebei province.
Key words： Yersinia pestis; Variable number of tandem repeat; Genotyping
WANG Hai-feng, YANG Shun-lin, ZHOU Song, BAI Xue-wei, ZHANG Yi-hui, YANG Xiao-yan, NIU Yan-fen, LIU He-zhi, DU Guo-yi, SHI Xian-ming . The application of tandem repeat in genetic typing Yersinia pestis of Hebei province[J]. Chinese Journal of Vector Biology and Control, 2016 , 27(2) : 141 -144 . DOI: 10.11853/j.issn.1003.8280.2016.02.012
[1] 刘合智,刘满福,李玉贵. 河北省鼠疫自然疫源地内自然染疫蚤的研究[J]. 中国媒介生物学及控制杂志,2005,16(3):206-208.
[2] 张晓媛. 中国鼠疫耶尔森菌多位点可变数目串联重复序列分析[D]. 北京:中国疾病预防控制中心,2008.
[3] 付秀萍,俞东征,海荣. 利用M28串联重复序列对鼠疫菌进行基因分型[J]. 疾病控制杂志,2006,10(1):21-23.
[4] Zhang XA, Hai R, Wei JC, et al. MLVA distribution characteristics of Yersinia pestis in China and the correlation analysis[J]. BMC Microbiol,2009,9:205.
[5] 崔玉军. 鼠疫耶尔森菌基因组多态性数据库及鉴定溯源系统的研究[D]. 北京:中国人民解放军军事医学科学院,2008.
[6] Li YJ,Cui YJ,Cui BZ,et al. Features of variable number of tandem repeats in Yersinia pestis and the development of a hierarchical genotyping scheme[J]. PLoS One,2013,8(6):e66567.
[7] Li YJ,Dai EH,Cui YJ,et al. Different region analysis for genotyping Yersinia pestis isolates from China[J]. PLoS One, 2008,3(5):e2166.
[8] 刘合智,张月芝,史献明,等. 河北省鼠疫菌株生化特性的研究[J]. 中国媒介生物学及控制杂志,2005,16(4):308-310.
[9] 董国润,王桂琴,白晓英,等. 115株鼠疫菌的生物学特性及流行病学研究[J]. 中国媒介生物学及控制杂志,2001,12(2):112-114.
[10] 王海峰,杨顺林,周松,等. 河北省鼠疫耶尔森菌多位点串联重复序列分析[J]. 中国媒介生物学及控制杂志,2015,26(2):141-144.
/
| 〈 |
|
〉 |