ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Comparison of biochemical characteristics of carboxylesterase between pyriproxyfen resistant and susceptible populations of Culex pipiens pallens
Received date: 2015-12-23
Online published: 2016-04-20
Supported by
Supported by the Municipal Natural Science Foundation of Shanghai of China (No. 12ZR1426600)
Objective To preliminarily explore biochemical resistance mechanism by comparison the biochemical characteristic of carboxylesterase in pyriproxyfen-resistantand susceptible population of Culex pipiens pallens. Methods The activity of carboxylesteres (CarE) was determined by van Aspern(1962). Results The tendency of CarE activity change in hydrolyzing α-naphthyl acetate (α-NA) or β-naphthyl acetate (β-NA) depended on substrate concentration. The hydrolyzing activity was significantly higher in resistant population than in susceptible population with α-NA and β-NA as substrates within the certain concentration range. When substrate was β-NA, the Kinetic parameters (Km) and Vmax of CarE were 27.20×10-5 mol/L and 115.00×10-5 A/(mg·pro·min) in resistance population respectively, and that of CarE in susceptible population were 104.00×10-5 mol/L and 207.00×10-5 A/(mg·pro·min). Difference between them was significant (tb=2.74, tc=3.16). When substrate was α-NA, there was no significant difference between Km (131.00×10-5 mol/L) in resistance population and Km (75.20×10-5 mol/L) in susceptible population; but difference was significant between Vmax of them. The CarE from resistant population was more sensitive to DDVP (dichlorvos) and propoxur than that from susceptible population. Conclusion Enhanced CarE activity maybe attributable to the resistance of Cx. pipiens pallens to pyriproxyfen.
LIU Hong-xia, XU Jin-qiu, LIU Yao, LENG Pei-en . Comparison of biochemical characteristics of carboxylesterase between pyriproxyfen resistant and susceptible populations of Culex pipiens pallens[J]. Chinese Journal of Vector Biology and Control, 2016 , 27(2) : 103 -106 . DOI: 10.11853/j.issn.1003.8280.2016.02.003
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