Objective To study the structure and feeding habits of Neoseiulus barkeri. Methods After oscillating and washing, the mite was killed with heated alcohol, then placed in the preservation solution. Mounting medium zishi was applied to the slide in the amount of 2-3 drops, and a cover glass was placed after adjusted mite posture with hair needle. The specimens were dried for identification under light microscope. Results Length of N. barkeri male body is about 310-350 μm, with light gray or light brown in color. Corniculus is long and slender, aggregated to center. The fixed digit, which has three tines, and movable digit, which has one denticulation, are approximately equal in length. The spermatodactyl is shap of "T". There are 17 pair setae on the back, all equal length except Z₅ which with comb shape and slightly longer. Outer edge hair r₃ and R₁ are located on the back. Besides anal seta there have other 4 pair setae. The female body length is about 370-390 μm. Color and shape is similar to those in male. The chelicerae is bigger than male's but the anal plate is smaller than male's. The egg is colorless and transparent, with oval shape, and size of (80-85) μm×(60-65) μm. Each female mite preyed Caloglyphus berlesei at an average of (12.80±1.47) per day and C. berlesei eggs with an average of (17.70±1.86) per day. When prey was absence, there was a tendency of self-mutilation. Conclusion We gained certain understanding of the morphology and feeding of N. barkeri, which provide a foundation for the further study.