ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Hard tick species identification and distribution in Aibi Lake located at China-Kazakhstan border
Received date: 2015-10-30
Online published: 2016-02-20
Supported by
Supported by the Administration of Quality Supervision, Inspection and Quarantine Science and Technology Project (No. 2014IK239), the National Science and Technology Pillar Program (No. 2013BAI05B05), Autonomous Region Natural Science Foundation of Xinjiang Uygur (No. 201442137-1), and the Special Fund for Agro-scientific Research in the Public Interest (No. 201103008)
Objective To investigate tick species and abundance in different sampling seasons, and explore the ticks' molecular characteristics in Aibi Lake, Alashankou Pass, Xinjiang Uygur Autonomous Region for providing the reference for the tick classification as well as prevention and control of tick-borne diseases. Methods During April to August in 2014, questing ticks were collected by dragging-flag method, and blood feeding ticks were collected from wild and domestic animals at Hejiaoke, Wulandabusen and the naturally preserve of Aibi Lake, Xinjiang, Northwest of China. The morphological and 16S rDNA identification were carried out based on representative ticks. Results We collected 434 ticks including 392 questing ticks and 42 blood feeding ticks, which including Hyalomma asiaticum, Dermacentor marginatus, D. niveus, Rhipicephalus sanguineus, Rh. turanicus, Rh. bursa and Haemaphysalis erinacei. The predominant tick species of Aibi Lake are H. asiaticum and D. marginatus. Their peak seasons were in May (Dermacentor) and June (Hyalomma). Blast analysis of 16S rDNA indicated that D. marginatus had 96%-100% identities compared with that of the ticks from Shihezi (KF547986), and that H. asiaticum was 98%-99% identical to Yili (KF527439), while Rh. sanguineus possessed 93%-94% identities with Israel (KF219732). Conclusion Rhipicephalus turanicus and Rh. bursa for the first time were found in Aibi Lake. 16S rDNA sequence of H. erinacei was reported for the first time, and was 91% identical to H. cretica. 16S rDNA of D. marginatus, H. asiaticum and Rh. sanguineus showed genetic divergence in Aibi Lake, which conveyed us that these tick species originated from different lineages.
Key words： Hard tick; Aibi Kake; Morphology; 16S rDNA
XU Jun, WANG An-dong, XIAO Yun-xia, XU Xin-long, DAI Li, DU Jing-yun, WANG Li-na, ZHANG Ke, LUO Dan, MU Lu-meng, ZHANG Ya-li, XING Jian-xin, WANG Yuan-zhi . Hard tick species identification and distribution in Aibi Lake located at China-Kazakhstan border[J]. Chinese Journal of Vector Biology and Control, 2016 , 27(1) : 21 -24 . DOI: 10.11853/j.issn.1003.4692.2016.01.006
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