Objective To master the current situation of infrastructure and service ability of rabies post-exposure prophylaxis (PEP) clinics in Qingdao. Methods All PEP clinics in municipal health institutions were investigated and over four-fifths in district or county-level institutions and more than one fourth in township health centers were randomly sampled. The contents included human resources, area settings, main necessary materials and facilities and service ability. Excel 2007 was used for data entry and Epi Info 6.04 for Chi-square test. Results There were 2 to 39 staff in each PEP clinic with the average of 12.54 of all, and part-timers accounted for 83.73%. The number ratio of doctors to nurses was 1:1.13. Surgeons and general practitioners totally constituted 90.85% of all doctors, nurses having licenses accounted for 93.07%. Of all clinics, 64.00% were within one department or single area, 100% had sterilizing alcohol and iodine tincture, 98.00% had emergency rescue medicine, 90.00% used a cold chain, 74.00% had a weight scale, 84.00% were open for 24 hours a day. All were able to treat the topical wounds and vaccinate, and 50.00% of which administered rabies immunoglobulin. The clinics in municipal health institutions were better than the other ones. Conclusion There were significant differences in infrastructure and service ability among rabies PEP clinics in different-level health institutions in Qingdao. The construction of some clinics in district, county-level and township health institutions should be strengthened.