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Chinese Journal of Vector Biology and Control >
The infection status of Rickettsia typhi in rodents on Heixiazi Island at the Sino-Russian Border
Received date: 2015-07-31
Online published: 2015-12-20
Supported by
Supported by the Project of Public Walfare Program(No. 201310072)and the National Special Project of International Cooperation in Science and Technology(No. 2012DFA30540)
Objective To study the infection status of Rickettsia typhi in rodents on Heixiazi Island at the Sino-Russian Border. Methods Rodents were captured by night trap on the Heixiazi Island between 2013 and 2014, liver tissues were processed by aseptic operation and the genomic DNA were extracted from them. Rickettsia typhi strains were confirmed by nest polymerase chain reaction (nest-PCR) of the 136 bp gltA gene. The positive amplification products were sequenced and compared with other R. typhi strains in GenBank, then phylogenetic analyses were drafted by software Mega 5.0. Results A total of 644 rodents were captured, which belong to 3 orders 5 family 8 genera. Apodemus agrarius which was the predominant species, accounting for 42.70% (275/644), followed by Clethrionomys rutilus (178/644, 27.64%) and Microtus fortis (136/644, 21.12%). The infection rate of R. typhi was 4.81%, with 31 rodent samples detected positive, which belong to 5 species. The infection rate of M. fortis (13/136, 9.56%) was relatively high, but no significant difference was found between the infection rate and the species of rodents by Fisher's exact probability (P=0.156). Although the infection rate of females (10/168, 5.95%) was higher than males (21/476, 4.41%), no significant difference of sex on the infection of R. typhi (χ2=0.643, P=0.423). There was no significant difference of weight on the infection of R. typhi (F=1.633, P= 0.202). However, there was significant difference of captured time on the infection of R. typhi (χ2=191.419, P=0.000). All the R. typhi in rodents on this region were in the same phylogenetic branch with a high similarity of above 99.98%,close with the USA strain CP003398 by the similarity above 95.83%. Conclusion The investigation proved that R. typhi is probably prevalent in rodents on Heixiazi Island at the Sino-Russian Border. The infection rate in rodents was significant difference in captured time. This study provides the basis for scientific development of Heixiazi Island in the future.
Key words： Rodents; Rickettsia typhi; Epidemiology; Heixiazi Island
LU Ting-ting, FU Ying-qun, HOU Yong, YANG Yu, XU Bao-liang, YING Chang-qing, CAI Jing, WANG Jian-cheng, ZHAO Ting-ting . The infection status of Rickettsia typhi in rodents on Heixiazi Island at the Sino-Russian Border[J]. Chinese Journal of Vector Biology and Control, 2015 , 26(6) : 590 -594 . DOI: 10.11853/j.issn.1003.4692.2015.06.013
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