ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Genetic polymorphism analysis on rNDA-ITS2 from different geographical strains of Culex pipiens pallens in Shandong province
Received date: 2015-08-21
Online published: 2015-12-20
Supported by
Supported by the National Natural Science Foundation of China(No. 81271877, 81471985)
Objective To explore gene sequence characteristics of rNDA-ITS2 in different populations of Culex pipiens pallens from Shandong province and analyze the genetic variation of these mosquitoes. Methods Adult-female mosquitoes were collected from different cities: Jinan, Jining, Qingdao, Liaocheng, Linyi, Zibo from Shandong and a sample from Nanjing. The pyrethroid-resistant and dichlorvos-resistant strains were reared in the laboratory. Single mosquito genomic DNA was extracted from Cx. pipiens pallens of these different populations and rNDA-ITS2 of Cx. pipiens pallens were specifically amplified by PCR. The PCR products were cloned into the pMD-19T simple vectors and sequenced. Genetic polymorphisms of them were analyzed by biological information systems. Results The obtained rDNA-ITS2 sequences were approximate 460 bp in length, with 36 mutation sites. Culex pipiens pallens from different geographical environment showed gene polymorphisms in sequence analysis of rNDA-ITS2. There were transition,transversion and deletion in the mono nucleotide of rNDA-ITS2 gene. Conclusion Genetic polymorphisms presented on rNDA-ITS2 of Cx. pipiens pallens from different geographical regions in Shandong province and different resistance strains in the lab were probably associated with the ecological environment.
Key words： Culex pipiens pallens; rDNA-ITS2; Genetic polymorphism
LIU Fei, GUO Xiu-xia, CHENG Peng, WANG Hai-fang, LIU Li-juan, YANG Pei-pei, ZHAO Yu-qiang, WANG Huai-wei, GONG Mao-qing . Genetic polymorphism analysis on rNDA-ITS2 from different geographical strains of Culex pipiens pallens in Shandong province[J]. Chinese Journal of Vector Biology and Control, 2015 , 26(6) : 550 -554 . DOI: 10.11853/j.issn.1003.4692.2015.06.003
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