ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Investigation on symbiotic bacteria (Wolbachia) in Aedes albopictus from central and eastern Zhejiang province, China
Received date: 2015-07-16
Online published: 2015-12-20
Supported by
Supported by the Major National Science and Technology Projects of China (No. 2012ZX10004219)
Objective To investigate the infection state and genotype of Wolbachia from Aedes albopictus in central and eastern Zhejiang province, and to provide a reference for mosquito and mosquito-borne disease control. Methods Light traps and human baits were applied to collect adult female mosquitoes, wsp genes of Wolbachia were tested by PCR and sequenced for phylogenetic analysis. Results In total 192 adult female mosquitoes were captured in six sampling sites: Hangzhou, Ningbo, Shengsi, Yiwu, Lishui and Wenzhou. The positive rate of wsp gene of Wolbachia was between 25.0% and 96.9% among these cities. There were 11 wsp haplotype sequences, of which lengths were between 581 bp and 605 bp, the average content of A, T, G and C was 31.4%, 31.1%, 21.5%, 16.0%, respectively. The homology of the nucleotide sequences of wsp was between 76.5% and 99.8%, and the homology of the amino acid sequences of wsp was between 66.2% and 100%. The 11 wsp haplotype sequences were divided in supergroup A (Wolbachia: wAlbA) and B (Wolbachia: wAlbB) by phylogenetic analysis. Conclusion Infection of Wolbachia in Ae. albopictus mosquitoes are widespread by in central and eastern Zhejiang province, whose genotypes belong to wAlbA and wAlbB.
Key words： Aedes albopictus; Symbiotic bacteria; Wolbachia; wsp gene
GUO Song, LING Feng, WANG Jin-na, WU Yu-yan, HOU Juan, GONG Zhen-yu . Investigation on symbiotic bacteria (Wolbachia) in Aedes albopictus from central and eastern Zhejiang province, China[J]. Chinese Journal of Vector Biology and Control, 2015 , 26(6) : 545 -549 . DOI: 10.11853/j.issn.1003.4692.2015.06.002
[1] Kittayapong P, Baisley KJ, Baimai V, et al. Distribution and diversity of Wolbachia infections in Southeast Asian mosquitoes (Diptera: Culicidae)[J]. J Med Entomol,2000,37(3): 340-345.
[2] Bian G, Xu Y, Lu P, et al. The endosymbiotic bacterium Wolbachia induces resistance to dengue virus in Aedes aegypti [J]. PLoS Pathogens,2010,6(4):e1000833.
[3] 潘晓玲,刘起勇,奚志勇. 基于昆虫共生菌沃尔巴克氏体的蚊媒和蚊媒病控制研究进展[J]. 中国媒介生物学及控制杂志, 2014,25(1):1-7.
[4] 郑小英,刘起勇,奚志勇. 基于沃尔巴克氏体的蚊媒和蚊媒病控制的生物安全性[J]. 中国媒介生物学及控制杂志,2014,25(2):93-96.
[5] Baldo L, Dunning Hotopp JC, Jolley KA, et al. Multilocus sequence typing system for the endosymbiont Wolbachia pipientis[J]. Appl Environ Microbiol,2006,72(11):7098-7110.
[6] 郑雅. Wolbachia诱导昆虫宿主细胞质不亲和的分子机理研究[D]. 武汉:华中师范大学,2012.
[7] Werren JH,Baldo L,Clark ME. Wolbachia: master manipulators of invertebrate biology[J]. Nat Rev Microbiol,2008,6(10): 741-751.
[8] 孟凤霞,王义冠,冯磊,等. 我国登革热疫情防控与媒介伊蚊的综合治理[J]. 中国媒介生物学及控制杂志,2015,26(1): 4-10.
[9] Zhou W, Rousset F, O'Neil S. Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences[J]. Proc R Soc Lond B,1998,265(1395):509-515.
[10] Kitrayapong P, Baimai V, O'Neill SL. Field prevalence of Wolbachia in the mosquito vector Aedes albopictus[J]. Am J Trop Med Hyg,2002,66(1):108-111.
[11] 杨筱慧,朱道弘,刘志伟,等. 湖南三地区麦氏安瘿蜂体内Wolbachia的感染及其wsp基因序列分析[J]. 昆虫学报, 2012,55(2):247-254.
[12] 林立丰,吴德,张欢,等. 广东省蚊虫感染沃尔巴克氏体初步调查研究[J]. 中国媒介生物学及控制杂志,2014,25(2): 113-115.
[13] Blagrove MS, Arias-Goeta C, Failloux AB, et al. Wolbachia strain wMel induces cytoplasmic incompatibility and blocks dengue transmission in Aedes albopictus[J]. Proc Natl Acad Sci USA,2012,109(1):255-260.
[14] 周健明,王德全,林琳,等. 深圳市龙岗区2009－2011年登革热媒介监测分析[J]. 中国公共卫生,2012,28(12): 1628-1630.
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