ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Studies on the unique repeat numbers of the VNTR in Chinese Francisella tularensis
Received date: 2015-06-29
Online published: 2015-12-20
Supported by
Supported by the National Natural Science Foundation of China (No. 81473032) and Major National Science and Technology Projects of China (No. 2012ZX10004219-007)
Objective To explore the genetic feature of Chinese Francisella tularensis using MLVA (multiple-locus variable number tandem repeat analysis). Methods Based on previous studies, 12 VNTR (variable number of tandem repeats) were selected that have higher resolving power on type B, and MLVA typing was conducted on 10 strains of Chinese F. tularensis and 29 reference strains that have been sequenced and published. Results The MLVA analysis indicated that Chinese F. tularensis has the unique copy numbers at the four VNTR: the copy number of repeat motif is 5 at the Ft-M3, and that is 1 at the Ft-M18, 2 at the Ft-M20, and 2 at the Ft-M21. Combined 12 VNTR analysis showed that the copy numbers of repeat motif were similar among some F. tularensis strains as follow:410108 and Japanese FSC022; 410112, 410113 and German F92; 410116, 410117 and American OSU18, Russian LVS. Conclusion By examining the 4 VNTR i.e. Ft-M3, Ft-M18, Ft-M20 and Ft-M21, a given isolate can be determined whether it is indigenous to China according to the unique copy numbers of repeat motif. In addition, the Chinese F. tularensis has higher genetic diversity comparing with F. tularensis from Europe, USA and Japan.
WANG Yan-hua, PENG Yao, XIA Lian-xu . Studies on the unique repeat numbers of the VNTR in Chinese Francisella tularensis[J]. Chinese Journal of Vector Biology and Control, 2015 , 26(6) : 541 -544 . DOI: 10.11853/j.issn.1003.4692.2015.06.001
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