Objective In order to assess the epidemic situation of hemorrhagic fever with renal syndrome (HFRS) after implementing expanded program on Immunization in Hulun Buir city, to provide a theoretical basis for the prevention and control of epidemic hemorrhagic fever. Methods We collected a crowd epidemic data and serological data and analyzed epidemiological characteristics of HFRS. Results Epidemic data showed the incidence of HFRS decreased in Hulun Buir city, but incidence showed an increasing trend in Daur Autonomous Banner of Morin Dawa. The HFRS had strong seasonality in 2009-2013. Onset age mainly between 20-64 years old. The farmer was 275 cases, accounting for 72.75%. Serological data results showed the positive rate of serum IgG antibody 47.17%, the recessive infection rate was 33.33%, in prevalence areas. Antibody positive rate and the recessive infection in females was higher than males, no statistically significant (χ²=2.290, P>0.05). The recessive infection rate of farmer was 7.86%. The density and virus-carrying rate of rat showed a rising trend year by year. Conclusion Results suggested continuation to strengthen emergency vaccination for high-risk groups in high prevalence areas, which will be conducive to further control the epidemic of HFRS.