Objective To observe the assessment for deratization effect used with rat footprint plate method and S100 check method, and comparison the accuracy of two methods. Methods On-the-spot test was carried out at the Zhangheitun village of Linyi city, a total of 280 households. It was divided into two groups, each group of 140 households, the first group put 0.05% Diphacine-Na corn poison bait in cement poison bait box, the second group put 0.05% Diphacine-Na wheat poison bait in. Two methods of rat footprint plate method and S400 check were used to evaluate the deratization effect at the same time. Results At the first group, the assessment of deratization rate with rat footprint plate method at different periods of time were 54.05%, 54.78%, 73.19% and 92.47% respectively on the 10 d, 20 d, 30 d and 60 d, and the assessment of deratization rate with S100 check method at different periods of time were 79.40%, 88.38%, 95.64% and 98.87% respectively on the 10 d, 20 d, 30 d and 60 d, comparison two assessment methods, they have statistically significant(10 d, χ²=71.540; 20 d, χ²=176.230; 30 d, χ²=181.730; 60 d, χ²=56.270, P<0.01). At the second group, the assessment of deratization rate with rat footprint plate method at different periods of time were 35.51%, 51.02%, 71.28% and 85.54% respectively on the 10 d, 20 d, 30 d and 60 d, and the assessment of deratization rate with S100 check method at different periods of time were 74.08%, 87.32%, 95.13% and 97.53% respectively on the 10 d, 20 d, 30 d and 60 d, comparison two assessment methods, they were statistically significant (10 d, χ²=145.720; 20 d, χ²=231.720; 30 d, χ²=214.560; 60 d, χ²=104.230, P<0.01). Conclusion The S100 check method is more suitable for the assessment of deratization effect, since its quality and accuracy. And the rat footprint plate method better suited to determine the population density of rats, and identifying the rat density situation, it was convenient and practical.