Objective Establishment of Aedes caspius laboratory breeding methods, to evaluate the resistance in Ae. caspius to commonly used insecticides in Kalamay. Methods Conventional breeding Ae. caspius and determined via the dosing method and median lethal dose method to detection F1 generation. Results Under conditions of temperature of (26±1) ℃ and the relative humidity of (40-60) %, the time of egg was 3 d, the time of larva was 7 d, the time of egg to emergence was 11 d, an egg batch resulted in about 110-120 larvae (does Ae. capius lay eggs singly). The LC₅₀ values of DDVP, propoxur, deltamethrin, β-cypermethrin, and biothion to larvae of Ae. caspius were 0.2838,2.7752,0.0024,0.0126, and 0.0033 mg/L respectively. Conclusion The LC₅₀ values for commonly used insecticides to Ae. caspius is similar to Ae. albopictus. Breeding place should be administered first in controlling Ae. caspius and insecticides should be used properly to avoid resistance development.