Analysis on a pathogenic strain of Yersinia enterocolitica in Dengfeng city, Henan, China

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  • 1 Dengfeng Hygiene and Anti-Epidemic Station, Dengfeng 452470, Henan Province, China;
    2 The People's Hospital of Dengfeng City;
    3 Henan Center for Disease Control and Prevention, Zhengzhou 450016, Henan Province, China

Received date: 2015-02-23

  Online published: 2015-08-20

Supported by

Supported by the Major National Science and Technology Projects of China(No. 2013ZX10004203-002-002)

Abstract

Objective To understand the strain of serotype, biotype, virulence genes, which cause infantile diarrhea. Methods Bacterial culture, serum agglutination method and PCR were used for serotyping, detection of virulence genes. Results A strain of Yersinia enterocolitica was isolated from the children of feces sample and the short bacillus of gram stain negative was identified under the microscope. Oxidase of the bacteria was negative, katalase was positive, no hydrogen sulfide production, urease test positive, ONPG test positive. The Y. enterocolitica strain is positive in O:3 serotyping test and it belongs to 3 biological types, carrying the virulence genes of ail+, ystA+, yadA+, virF+ but no ystB- . Conclusion The case of infantile diarrhea disease is caused by pathogenic Y. enterocolitica.

Cite this article

ZHANG Sheng-yong, MEI Zhi-min, LI Hong-min, ZHANG Jin, WANG De-xiang, XIA Sheng-li . Analysis on a pathogenic strain of Yersinia enterocolitica in Dengfeng city, Henan, China[J]. Chinese Journal of Vector Biology and Control, 2015 , 26(4) : 409 -410 . DOI: 10.11853/j.issn.1003.4692.2015.04.021

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