ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Molecular characteristics of emerging spotted fever group Rickettsiae and Anaplasma phagocytophilum in Hebei province, China
Received date: 2015-03-10
Online published: 2015-08-20
Supported by
Supported by the National Basic Research Program of China(973 Program)(No. 2010CB530206)and the National Key Science and Technology Projects of China(No. 2008ZX10004-008, 2012ZX10004215)
Objective To better understand the molecular epidemiological characteristics of emerging spotted fever group Rickettsiae and Anaplasma phagocytophilum identified in Hebei province. Methods One hundred and one clinical probable cases of typhus were collected from Xinji city, Qianan city and Dingzhou city, Hebei province during 2009-2012 and 101 acute stage and 17 convalescence blood samples were collected. Sera separated from blood were used for detecting the IgM and IgG antibodies against Rickettsiae prowazekii, R. typhi, R. felis, Bartonella henselae, Orientia tsutsugamushi, R. heilongjiangensis, R. sibirica, Ehrlichia chaffeensis, Coxiella burnetii, Chinese spotted fever group rickettsia Hainan-1 and Chinese A. phagocytophilum strain CZ-HGA-2 and LZ-HGA-3, respectively. Two sets of nested PCR, which targeting the A. phagocytophilum 16S rRNA gene and spotted fever group rickettsiae groEL gene respectively, were conducted using the acute stage blood DNAs of patients as temples. Sequences were analyzed by DNAStar MegAlign. Results PCR positive rates were 10.9%(11/101)for amplifying groEL gene and two genetic groups of spotted fever group rickettsiae were identified. Although the sequence analysis failed to differentia the emerging spotted fever group rickettsiae from traditional ones because of limited PCR fragments, the sera from the patients with positive PCR did not reactive with the R. heilongjiangensis, R. sibirica, R. felis, and Chinese spotted fever group ricketsia Hainan-1, which indicated that the emerging spotted fever group rickettsiae might be prevalence in these areas. PCR positive rates of A. phagocytophilum were 8.9% (9/101) and all of the 9 patients with positive PCR were found to be positive of the IgM antibodies against A. phagocytophilum and 3 patients had IgG seroconversation (4-fold increase of IgG antibody titer). The 9 sequences of the 16S rRNA genes of A. phagocytophilum were not only 100% identity with each other but 100% identity with the local A. phagocytophilum strain CZ-HGA-2. Conclusion Emerging tick-borne rickettsiae were highly prevalence in Hebei province and further etiological investigations were urgently needed.
Key words： Emerging spotted fever; Anaplasomoses; Rickettsiae
SUN Yin-qi, WANG Yong, JIANG Xia, YAO Na, QIAN Zhen-yu, LIU Xiao-li, CHEN Chuang-fu, ZHANG Li-juan . Molecular characteristics of emerging spotted fever group Rickettsiae and Anaplasma phagocytophilum in Hebei province, China[J]. Chinese Journal of Vector Biology and Control, 2015 , 26(4) : 344 -348 . DOI: 10.11853/j.issn.1003.4692.2015.04.004
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