ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Genetic polymorphism analysis on mtDNA-COⅠ of Aedes albopictus in Shenzhen, China
Received date: 2015-05-01
Online published: 2015-08-20
Supported by
Supported by the Medical Research Foundation in Guangdong Province(No. B2013356)
Objective To study the characteristics of mitochondrial DNA cytochrome oxidase subunitⅠ (mtDNA-COⅠ) gene and the spatial population genetic diversity of Aedes albopictus in Shenzhen. Methods Larvae of Ae. albopictus were collected from eight investigation sites in Longgang, Guangming, Futian,Dapeng, Luohu, Longhua, Nanshan and Baoan district, and reared in the laboratory, and the resulted adults were preserved in 75% alcohol under -20 ℃. Single mosquito genomic DNA was extracted from Ae. albopictus of different populations, and mtDNA-COⅠ gene were specifically amplified by PCR. The amplified mtDNA-COⅠ gene sequences in different geographic strains were analyzed and compared with the others in the GenBank, and genetic polymorphism analysis of them was conducted by bio-information system. Results The obtained COⅠ sequences of Ae. albopictus of eight different isolates from Shenzhen showed 99% homogeny with 1433 bp in length. As there were transition and transversion in the mononucleotide of mtDNA-COⅠ gene, Ae. albopictus from different geographical locations in Shenzhen showed gene polymorphism in sequence analysis on mtDNA-COⅠ with 24 mutation sites and 1.67% mutation rate. However, there was no difference in gene sequence of mtDNA-COⅠ in the five mosquitoes of Ae. albopictus from the same geographical location. Conclusion The mtDNA-COⅠ gene of Ae. albopictus from different collection sites of Shenzhen have high homogeny, but single nuclear polymorphism(SNP) was also observed among different Ae. albopictus populations. The SNP forming reason needs to be further studied.
YANG Fan, ZHANG Ren-li, LIU Yang, ZHANG Shao-hua, HUANG Da-na, WU Chun-li, LI Yue, TANG Yi-jun . Genetic polymorphism analysis on mtDNA-COⅠ of Aedes albopictus in Shenzhen, China[J]. Chinese Journal of Vector Biology and Control, 2015 , 26(4) : 337 -340 . DOI: 10.11853/j.issn.1003.4692.2015.04.002
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