ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Morphological classification and molecular identification of Rhipicephalus turanicus in Yining county, Xinjiang Autonomous Region, China
Received date: 2014-12-15
Online published: 2015-06-20
Supported by
Supported by the Grants from the International Cooperation in Science and Technology(No. 2010CB530303), the National Science and Technology Pillar Program(No. 2013BAI05B05), and National Natural Science Foundation of China(No. 31060334)
Objective To identify Rhipicephalus turanicus in Yining county, Xinjiang Autonomous Region based on morphological and molecular evidence. Methods In total, 324 ticks were collected off livestock in the border areas of Yining county, Xinjiang Autonomous Region. All ticks collected were initially screened according to morphological features, and then 6 ticks were selected by morphological differences, then the 16S rRNA and COⅠ were amplified by PCR and subsequently sequenced, and phylogenetically analyzed with GenBank sequences. Results Rhipicephalus turanicus was observed and analyzed for its morphological characteristics. A high percentage of A+T base composition in the two sequences was observed, variation on the 16S rRNA gene was significantly conservative than COⅠ gene. Conclusion Rhipicephalus turanicus were firstly reported by morphological, 16S rRNA and COⅠ gene sequencing analysis, and it present high biodiversity in Yining county, Xinjiang.
DU Jing-yun, MU Lu-meng, ZHANG Lin, WANG Li-na, WANG An-dong, ZHANG Ke, CHEN Chuang-fu, WANG Yuan-zhi . Morphological classification and molecular identification of Rhipicephalus turanicus in Yining county, Xinjiang Autonomous Region, China[J]. Chinese Journal of Vector Biology and Control, 2015 , 26(3) : 271 -274 . DOI: 10.11853/j.issn.1003.4692.2015.03.013
[1] 杨晓军,陈泽,刘敬泽. 中国蜱类的有效属和有效种[J]. 河北师范大学学报:自然科学版,2008,32(4):529-533.
[2] 陈泽.中国蜱类名录订正、区系分析及扇头蜱亚科Rhipicephalinae 部分种类的生物学特性研究[D]. 石家庄:河北师范大学, 2007.
[3] 刘明社,黄克峻,赵中夫,等. 9省区蜱类区系初步调查[J]. 长治医学院学报,2005,19(4):249-250.
[4] 吕新军,唐青,冯育明,等. 新疆南部地区蜱传虫媒病毒分子生物学调查[J]. 中华实验和临床病毒学杂志,2005,19(4): 325-330.
[5] Satta G,Chisu V,Cabras P,et al. Pathogens and symbionts in ticks:a survey on tick species distribution and presence of tick- transmitted micro - organisms in Sardinia,Italy[J]. J Med Microbiol,2011,60(1):63-68.
[6] Aktas M,Vatansever Z,Ozubek S,et al. Molecular evidence for trans - stadial and transovarial transmission of Babesia occultans in Hyalomma marginatum and Rhipicephalus turanicus in Turkey[J]. Vet Parasito,2014,204(3/4):369-371.
[7] Razmi G,Yaghfoori S. Molecular surveillance of Theileria ovis, Theileria lestoquardi and Theileria annulata infection in sheep and ixodid ticks in Iran[J]. Onderstepoort J Vet Res,2013,80 (1):635.
[8] 张璘,王远志,陈创夫,等. 新疆北疆部分地区硬蜱伯氏疏螺旋体基因型研究[J]. 中华流行病学杂志,2014,35(3):39-42.
[9] 邓国藩,姜在阶. 中国经济昆虫志. 第39册. 蜱螨亚纲. 硬蜱科[M]. 北京:科学出版社,1991:62-326.
[10] 王启果,张渝疆,孙素荣. DNA序列应用于蜱类分子系统发育学的研究进展[J]. 疾病预防控制通报,2012,27(2):83-86.
[11] Dantas-Torres F,Latrofa MS,Annoscia G,et al. Morphological and genetic diversity of Rhipicephalus sanguineus sensu lato from the New and Old Worlds[J]. Parasit Vectors,2013,6:213-229.
[12] Black WC,Piesman J. Phylogeny of hard and soft-tick taxa (Acari: Ixodidae) based on mitochondrial 16S DNA sequences[J]. Proc Natl Acad Sci USA,1994,91(21):10034-10038.
[13] 热孜万·阿不力孜,于心, 阿布力克木·阿不都热西提. 塔里木盆地三种扇头蜱的鉴别要点[J]. 地方病通报,2007,22(2):4-5.
[14] 叶瑞玉,于心,陈饮如,等. 新疆南部地区蜱螨区系及医学意义[J]. 地方病通报,1995,10(4):30-34.
[15] Murrell A,CamPbell NJ,Barker SC. Phylogenetic analyses of the rhipicephaline ticks indicate that the genus Rhipicephalus is paraphyletic[J]. Mol Phylogenet Evol,2000,16(1):1-7.
[16] Gylfe A,Yabuki M,Drotz M,et al. Phylogeographic relationships of Ixodes uriae (Acari:Ixodidae) and their significance to transequatorial dispersal of Borrelia garinii[J]. Hereditas,2001, 134(3):195-199.
[17] Caporale DA,Rich SM,Spielman A,et al. Discriminating between Ixodes ticks by means of mitochondrial DNA sequences[J]. Mol Phylogenet Evol,1995,4(4):361-365.
[18] McManus DP, Bowles J. Molecular genetic approaches to parasite identification:their value in diagnostic parasitology and systematics[J]. Int J Parasitol,1996,26(7):687-704.
[19] 吕继洲,吴绍强,张永宁,等. 小亚璃眼蜱、亚洲璃眼蜱和残缘璃眼蜱的分子生物学鉴定[J]. 中国畜牧兽医,2013,40(6): 7-13.
[20] 戴艳菊,刘萍,高保全,等. 三疣梭子蟹4个野生群体线粒体 16S rRNA和COⅠ基因片段的比较分析[J]. 中国海洋大学学报,2010,40(3):54-60.
[21] Switzer WM,Salemi M,Shanmugam V,et al. Ancient co- speciation of simian foamy viruses and primates[J]. Nature, 2005,434(7031):376-380.
/
| 〈 |
|
〉 |